{"id":"W4407268589","doi":"10.1038/s41598-025-88861-0","title":"Distinct patterns of prion strain deposition and toxicity in a novel whole brain organotypic slice culture system","year":2025,"lang":"en","type":"article","venue":"Scientific Reports","topic":"Prion Diseases and Protein Misfolding","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Women and Children’s Health Research Institute; University of Alberta","funders":"Creutzfeldt-Jakob Disease Foundation; Alberta Innovates Bio Solutions","keywords":"Scrapie; Biology; Transmissible spongiform encephalopathy; Prion protein; Phenotype; Tropism; In vivo; Slice preparation; Cell biology; Virology; Disease; Neuroscience; Pathology; Central nervous system; Medicine; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006339224,0.0009280554,0.0006838546,0.0004774787,0.0004071129,0.0008072885,0.0009786004,0.0006278313,0.0009482115],"category_scores_gemma":[0.0002340644,0.0004900671,0.0007041734,0.0003636293,0.0005214004,0.0005043869,0.0005453333,0.001443326,0.001171064],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004379406,"about_ca_system_score_gemma":0.0006232113,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003323704,"about_ca_topic_score_gemma":0.01168957,"domain_scores_codex":[0.9995317,0.00005851695,0.00007856359,0.0001449812,0.0001250972,0.00006119507],"domain_scores_gemma":[0.9994203,0.0001431273,0.0001142432,0.0001108668,0.0001165212,0.00009502163],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003022018,0.00002060527,0.00009633794,0.00004499274,0.000009427959,0.00007953047,0.00003909104,0.00006864539,0.9992211,0.00007792159,0.0000338067,0.0002782258],"study_design_scores_gemma":[0.0000253057,0.0005139518,0.005137812,0.00003532768,0.0001065388,0.00115407,0.00009574729,0.002257365,0.9874842,0.0001578293,0.003002519,0.00002929067],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8134301,0.00662877,0.1663413,0.0004029142,0.0004696872,0.0004721472,0.005558078,0.0008841964,0.005812765],"genre_scores_gemma":[0.7439265,0.009753339,0.2287642,0.000284321,0.0001002077,0.0008892622,0.007063679,0.0003124621,0.008905965],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003323704,"threshold_uncertainty_score":0.006608725,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005238690359842796,"score_gpt":0.2368483386679568,"score_spread":0.231609648308114,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}