{"id":"W4407386591","doi":"10.1002/ece3.70996","title":"Droplet Digital <scp>PCR</scp> Provides Highly Sensitive and Accurate Opsin Gene <scp>SNP</scp> Detection From Wild Primate Fecal Samples","year":2025,"lang":"en","type":"article","venue":"Ecology and Evolution","topic":"Single-cell and spatial transcriptomics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Alberta Children's Hospital; University of Calgary","funders":"Japan Society for the Promotion of Science; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Sanger sequencing; Biology; Feces; Digital polymerase chain reaction; Genotype; Single-nucleotide polymorphism; Genetics; Allele; Population; Polymerase chain reaction; Zoology; DNA sequencing; Gene; Ecology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001788377,0.0005278941,0.0004621404,0.001030167,0.0003738895,0.00103828,0.0005241672,0.000663537,0.003997545],"category_scores_gemma":[0.002704841,0.0003434193,0.0003266216,0.0006151012,0.0007773645,0.0004875606,0.0006036507,0.0007098764,0.002895429],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003787424,"about_ca_system_score_gemma":0.0004161343,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001082903,"about_ca_topic_score_gemma":0.003514697,"domain_scores_codex":[0.9978735,0.0004330325,0.0001153694,0.0007949191,0.0006817286,0.0001014347],"domain_scores_gemma":[0.9979869,0.0006377911,0.0004463764,0.0003432599,0.000505065,0.0000806069],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001240022,0.00007598646,0.01656713,0.0003080109,0.0000612006,0.0001432485,0.0001420535,0.0006836735,0.9325467,0.0006517943,0.002895666,0.04580052],"study_design_scores_gemma":[0.00002953339,0.0002671538,0.06327257,0.00009482356,0.0000744831,0.0009168654,0.0001691842,0.01807977,0.8881504,0.001157013,0.02773016,0.00005813238],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5080678,0.002771104,0.449782,0.0009128153,0.0003661717,0.0007140098,0.01695916,0.006722624,0.01370441],"genre_scores_gemma":[0.6612784,0.0009473525,0.3182734,0.001157661,0.000144087,0.0006290881,0.007933655,0.0007233133,0.00891308],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003997545,"threshold_uncertainty_score":0.01337308,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008898633439651934,"score_gpt":0.2173467139878199,"score_spread":0.208448080548168,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}