{"id":"W4407421670","doi":"10.1002/open.202400340","title":"Measuring the Thermal Unfolding of Lysozyme: A Critical Comparison of Differential Scanning Fluorimetry and Differential Scanning Calorimetry","year":2025,"lang":"en","type":"article","venue":"ChemistryOpen","topic":"Protein Structure and Dynamics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba","funders":"Natural Sciences and Engineering Research Council of Canada; Deutsche Forschungsgemeinschaft","keywords":"Differential scanning calorimetry; Enthalpy; Chemistry; Dynamic light scattering; Fluorescence spectroscopy; Lysozyme; Thermodynamics; Folding (DSP implementation); Circular dichroism; Equilibrium unfolding; Heat capacity; Crystallography; Analytical Chemistry (journal); Fluorescence; Materials science; Chromatography; Nanoparticle; Nanotechnology; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001409552,0.0002064728,0.0003639313,0.00003911776,0.0001685934,0.00003986727,0.0003976629,0.0002056929,0.00002243301],"category_scores_gemma":[0.0003220364,0.0001653234,0.000116501,0.0001411204,0.0003736836,0.000006519884,0.000500259,0.0002124238,1.817782e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000157498,"about_ca_system_score_gemma":0.00008813542,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004755174,"about_ca_topic_score_gemma":0.000002757095,"domain_scores_codex":[0.9987739,0.00004744056,0.0003955371,0.0003303832,0.0001955153,0.0002572114],"domain_scores_gemma":[0.9992154,0.00007061931,0.0001434059,0.0003920427,0.0001147927,0.00006368693],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000128148,0.00004019908,0.02574924,0.00024506,0.000128202,6.469698e-7,0.00009268496,0.00002386099,0.9709797,0.0002175211,0.00006893426,0.002325836],"study_design_scores_gemma":[0.0005125561,0.00004000998,0.01195311,0.0001535024,0.0001089559,0.000004063151,0.0002075587,0.0006197809,0.9860848,0.00008574147,0.0000727925,0.000157127],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9893408,0.001688424,0.007022248,0.00004647265,0.0001814575,0.0001347949,0.00001572206,0.000008659246,0.001561407],"genre_scores_gemma":[0.9992467,0.00001153974,0.0003934172,0.00001906163,0.0001699865,0.000009854323,0.00003356175,0.00001613196,0.00009981907],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01510514,"threshold_uncertainty_score":0.6741693,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01409314535435309,"score_gpt":0.2904362963417667,"score_spread":0.2763431509874136,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}