{"id":"W4407844539","doi":"10.1016/j.xpro.2025.103649","title":"Protocol for the purification and crystallization of the Drosophila melanogaster Cfp1PHD domain in complex with an H3K4me3 peptide","year":2025,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Genomics and Chromatin Dynamics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Drosophila melanogaster; Crystallization; Domain (mathematical analysis); Protocol (science); H3K4me3; Computational biology; Cell biology; Biology; Chemistry; Biochemistry; Medicine; Mathematics; Pathology; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002149575,0.00009091789,0.00008639113,0.00001913704,0.00008668558,0.00002843251,0.0001927714,0.00005772649,0.000003589982],"category_scores_gemma":[0.00001411968,0.00005263423,0.0000252782,0.00007109364,0.00009535392,0.000004055302,0.00006152744,0.00004104257,8.077388e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001197645,"about_ca_system_score_gemma":0.0000802482,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00000860122,"about_ca_topic_score_gemma":0.0002358397,"domain_scores_codex":[0.9994002,0.00005726254,0.0001887121,0.0001830568,0.00006722412,0.0001035169],"domain_scores_gemma":[0.9994314,0.00001148505,0.000114072,0.0003545091,0.00007410135,0.00001438422],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"observational","study_design_scores_codex":[0.001630407,0.0003191827,0.01863698,0.0008094966,0.00006959624,2.634275e-7,0.0004720141,0.0009907025,0.9633834,0.01096416,0.0005833602,0.002140462],"study_design_scores_gemma":[0.02014745,0.004543481,0.3611545,0.0007706646,0.00006619441,0.00001505514,0.001401386,0.0259834,0.187865,0.03609741,0.3610747,0.0008808261],"study_design_candidate":"bench_or_experimental","study_design_consensus":null,"genre_codex":"protocol","genre_gemma":"protocol","genre_scores_codex":[0.1392469,0.00000226114,0.009728797,0.0004551756,0.000005791258,0.8501723,0.00003590186,0.000004354077,0.0003484825],"genre_scores_gemma":[0.1143211,3.239568e-7,0.004790819,0.00009567709,0.00002060074,0.8805984,0.00003329281,0.00001113866,0.0001286213],"genre_candidate":"protocol","genre_consensus":"protocol","teacher_disagreement_score":0.7755184,"threshold_uncertainty_score":0.2146362,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02093970793423782,"score_gpt":0.3078199377821858,"score_spread":0.286880229847948,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}