{"id":"W4408486376","doi":"10.1101/2025.03.12.642913","title":"Engineering Conjugative Plasmids for Inducible Horizontal DNA Transfer","year":2025,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"","keywords":"Plasmid; Horizontal gene transfer; Biology; Gene; Biotechnology; CRISPR; Antimicrobial; Computational biology; Gene transfer; Genetics; Biochemical engineering; Microbiology; Genome; Engineering","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007121782,0.0006804065,0.0003629958,0.0005638287,0.0001589724,0.000594775,0.0004036999,0.0003925783,0.001642301],"category_scores_gemma":[0.0007746912,0.00023882,0.0003672057,0.000465722,0.000328547,0.0002974028,0.0005852188,0.001167084,0.0009721117],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000402293,"about_ca_system_score_gemma":0.000356897,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003318416,"about_ca_topic_score_gemma":0.0003185697,"domain_scores_codex":[0.9994344,0.0001453623,0.00005457027,0.00009613408,0.0001861469,0.00008342753],"domain_scores_gemma":[0.9996294,0.0001262918,0.0001022796,0.00006856331,0.00003975803,0.0000336822],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000499217,0.00007247677,0.0003857196,0.00007898395,0.00001316894,0.00008708401,0.00003383614,0.0002942726,0.9921956,0.0006668394,0.0001369461,0.005985145],"study_design_scores_gemma":[0.00001913682,0.0001759131,0.0006501246,0.000007538032,0.00001637997,0.0001927291,0.00001695103,0.001462252,0.9925681,0.0001717167,0.004711518,0.000007561821],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7203838,0.001071792,0.2707336,0.0003871295,0.0001609916,0.0007149225,0.001333213,0.001547688,0.003666827],"genre_scores_gemma":[0.9038991,0.0007065321,0.08670994,0.00008250526,0.00002504332,0.0002359402,0.002197585,0.0004155479,0.005727838],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001642301,"threshold_uncertainty_score":0.005493999,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008417646701036974,"score_gpt":0.2432479778711372,"score_spread":0.2348303311701002,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}