{"id":"W4408492424","doi":"10.1094/pdis-01-25-0003-sr","title":"Development of a Triplex Quantitative PCR System for the Detection of <i>Parastagonospora nodorum</i> , <i>Zymoseptoria tritici</i> , and <i>Pyrenophora tritici-repentis</i> from Wheat","year":2025,"lang":"en","type":"article","venue":"Plant Disease","topic":"Plant Disease Resistance and Genetics","field":"Agricultural and Biological Sciences","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan; Alberta Health; Agriculture and Agri-Food Canada; Agriculture Food and Rural Development","funders":"","keywords":"Pyrenophora; Biology; Septoria; Mycosphaerella graminicola; Polymerase chain reaction; Primer (cosmetics); Population; Multiplex polymerase chain reaction; Genetics; Botany; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001970496,0.001701517,0.001713623,0.001203319,0.0004892813,0.0008165781,0.001534759,0.0009455263,0.002182],"category_scores_gemma":[0.001302732,0.0009560484,0.001020519,0.0007885972,0.0007628927,0.0005966941,0.0007978935,0.001732962,0.00147901],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007491159,"about_ca_system_score_gemma":0.001143756,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001771001,"about_ca_topic_score_gemma":0.004055255,"domain_scores_codex":[0.9973003,0.0003228412,0.0001729649,0.001122391,0.0008347911,0.0002466435],"domain_scores_gemma":[0.9988725,0.0003375936,0.0001833133,0.0001355835,0.0003688421,0.0001022244],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004880503,0.00004216475,0.0002087166,0.00008163629,0.000007482995,0.00001479595,0.0000520764,0.0001149704,0.9955278,0.00009366593,0.00009687137,0.003711064],"study_design_scores_gemma":[0.00003312957,0.000775762,0.002833339,0.00002957216,0.00007249468,0.0002019597,0.00005968495,0.008889322,0.9821842,0.000139933,0.004732391,0.0000482571],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1957553,0.0006030833,0.7877172,0.0001964645,0.0002637914,0.002244052,0.005319677,0.005062298,0.002838166],"genre_scores_gemma":[0.1650203,0.0008375887,0.8097316,0.0003998109,0.00006342951,0.005305502,0.008372752,0.0004779279,0.009790989],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002182,"threshold_uncertainty_score":0.0104211,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01980609292703938,"score_gpt":0.2310065448623382,"score_spread":0.2112004519352988,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}