{"id":"W4408571284","doi":"10.3390/v17030437","title":"Adapting Next-Generation Sequencing to in Process CRISPR-Cas9 Genome Editing of Recombinant AcMNPV Vectors: From Shotgun to Tiled-Amplicon Sequencing","year":2025,"lang":"en","type":"article","venue":"Viruses","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"CRISPR; Biology; Cas9; Autographa californica; Genome editing; Amplicon; Genome; Subgenomic mRNA; Computational biology; Shotgun; Sf9; Genetics; Recombinant DNA; Gene; Virology; Spodoptera; Polymerase chain reaction","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002054596,0.0007934342,0.0009293181,0.0008999166,0.0004477311,0.001773616,0.0007564616,0.000803237,0.001058026],"category_scores_gemma":[0.002074563,0.0008027251,0.000778033,0.0005784555,0.0004813353,0.0006555848,0.001274937,0.00235678,0.001326608],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006203357,"about_ca_system_score_gemma":0.0008042001,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001388053,"about_ca_topic_score_gemma":0.004122658,"domain_scores_codex":[0.9989032,0.000155587,0.00009232194,0.0003485487,0.0003964968,0.0001038475],"domain_scores_gemma":[0.9990128,0.0002720269,0.0001380936,0.0002193844,0.0002601056,0.00009744086],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000114666,0.00005071692,0.001156593,0.000272822,0.00007154825,0.000154325,0.000210306,0.001669856,0.9593646,0.001213693,0.0008831141,0.03483772],"study_design_scores_gemma":[0.00003167456,0.0002615343,0.004090487,0.00007404557,0.00008642417,0.0005123655,0.0001368843,0.0273491,0.9323871,0.002680796,0.03228522,0.0001043814],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.12707,0.00199709,0.8542606,0.0003968689,0.0002995139,0.0007374611,0.003404799,0.007308065,0.004525625],"genre_scores_gemma":[0.1185891,0.001562323,0.868213,0.0003868482,0.00004409937,0.0005090706,0.00605986,0.001487754,0.003147867],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002054596,"threshold_uncertainty_score":0.01086593,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07426223361428597,"score_gpt":0.3284823653836831,"score_spread":0.2542201317693971,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}