{"id":"W4408644416","doi":"10.1007/978-1-0716-4358-7_2","title":"Accelerated Laboratory Evolution of Conjugative Plasmids for CRISPR Delivery in Bacteria","year":2025,"lang":"en","type":"book-chapter","venue":"Springer protocols handbooks/Springer protocols","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université de Sherbrooke","funders":"","keywords":"CRISPR; Plasmid; Bacteria; Biology; Computational biology; Genetics; Microbiology; DNA; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007367598,0.0007212263,0.0006416946,0.0008353267,0.0003567153,0.001112589,0.001072562,0.0007252321,0.007913204],"category_scores_gemma":[0.0005280697,0.0005260572,0.0005761665,0.0008611857,0.0003470942,0.0009234279,0.001215896,0.002472134,0.00782852],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007675607,"about_ca_system_score_gemma":0.0005600621,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005052559,"about_ca_topic_score_gemma":0.001072988,"domain_scores_codex":[0.9994289,0.00008497619,0.00003163939,0.00008930909,0.0003096058,0.00005539366],"domain_scores_gemma":[0.9997585,0.00007069681,0.00002599161,0.00007178116,0.00004581411,0.00002729322],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008276368,0.00008743296,0.000106822,0.0003965824,0.00001769739,0.0001158863,0.0001001972,0.00105982,0.8081086,0.01631292,0.01107233,0.1625389],"study_design_scores_gemma":[0.00002679033,0.0002074124,0.000616684,0.00007955037,0.00002833881,0.0006730783,0.00002760616,0.003399302,0.7558705,0.004177465,0.2348483,0.00004493314],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.09711233,0.03108588,0.7444473,0.001598858,0.001588717,0.0006176202,0.003082814,0.01187446,0.108592],"genre_scores_gemma":[0.2064444,0.02859177,0.5040476,0.0009140545,0.0002934299,0.000970712,0.007676581,0.003831538,0.24723],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.007913204,"threshold_uncertainty_score":0.02647227,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02210713138760932,"score_gpt":0.331061220958909,"score_spread":0.3089540895712997,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}