{"id":"W4408784608","doi":"10.1186/s12866-025-03882-z","title":"A one-pot method for universal Dengue virus detection by combining RT-RPA amplification and CRISPR/Cas12a assay","year":2025,"lang":"en","type":"article","venue":"BMC Microbiology","topic":"Mosquito-borne diseases and control","field":"Medicine","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Infection and Immunity","funders":"National Key Research and Development Program of China; National Natural Science Foundation of China; Natural Science Foundation of Shanghai","keywords":"Biology; CRISPR; Dengue virus; Dengue fever; Virology; Parasitology; Computational biology; Genetics; Gene; Zoology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003087304,0.0001285481,0.0003003445,0.0001090192,0.0001180587,0.00001636669,0.00006825275,0.0001808473,0.00002969386],"category_scores_gemma":[0.0002100669,0.0001306075,0.00009209335,0.0001066882,0.00005397101,0.00003114702,0.00003483292,0.0001136423,0.000006627626],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00007321185,"about_ca_system_score_gemma":0.00009066418,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001756683,"about_ca_topic_score_gemma":0.0002125063,"domain_scores_codex":[0.9989815,0.0001843763,0.0002130996,0.0003703771,0.00002349885,0.0002271205],"domain_scores_gemma":[0.9991512,0.0003809918,0.00008893216,0.0002094161,0.0001030788,0.0000663634],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000698527,0.0001058523,0.00106189,0.0001103685,0.0001104832,7.850849e-7,0.00004406066,0.000001948266,0.9695264,0.001439228,0.002501054,0.02439943],"study_design_scores_gemma":[0.02567872,0.001682609,0.02461961,0.0002652446,0.002904132,0.0001507163,0.001596635,0.01386624,0.6564452,0.001944393,0.2699646,0.0008818766],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.1700102,0.001594859,0.8262036,0.0005980206,0.0002778937,0.0008656899,0.0001511024,0.00007338313,0.000225291],"genre_scores_gemma":[0.9910582,0.0000457533,0.006635246,0.0009730437,0.00007179044,0.00009227815,0.0002752804,0.0000163232,0.0008321129],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.821048,"threshold_uncertainty_score":0.5326021,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0151779267267722,"score_gpt":0.3056023812636539,"score_spread":0.2904244545368817,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}