{"id":"W4408867088","doi":"10.1083/jcb.202408158","title":"An advanced toolset to manipulate and monitor subcellular phosphatidylinositol 3,5-bisphosphate","year":2025,"lang":"en","type":"article","venue":"The Journal of Cell Biology","topic":"Calcium signaling and nucleotide metabolism","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia; University of Victoria; Children’s Health Research Institute; Western University","funders":"Eunice Kennedy Shriver National Institute of Child Health and Human Development; National Institute of Child Health and Human Development; Natural Sciences and Engineering Research Council of Canada; National Institutes of Health; Michael Smith Health Research BC","keywords":"Dictyostelium discoideum; Phosphatidylinositol; Cell biology; Pi; Endocytic cycle; Subcellular localization; Biochemistry; Biology; Chemistry; Cell; Signal transduction; Gene; Endocytosis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004471627,0.0001443686,0.000233766,0.00006353403,0.00009653234,0.00001956345,0.0003090592,0.0001176646,0.00001150108],"category_scores_gemma":[0.00004647898,0.00009831579,0.0000722999,0.00009841943,0.00007901483,0.000006737662,0.00008689294,0.0001423968,0.000004170051],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000007924807,"about_ca_system_score_gemma":0.00004458101,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001285201,"about_ca_topic_score_gemma":0.000003444611,"domain_scores_codex":[0.9990701,0.0001674662,0.0003294301,0.000160227,0.00006121584,0.000211559],"domain_scores_gemma":[0.9993008,0.00003865826,0.0001664074,0.0002436326,0.0001429849,0.0001075297],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005961353,0.00003856236,0.0006790771,0.000008640312,0.00004038743,0.000003208212,0.0000494976,0.0002098726,0.9902204,0.0001128845,0.0001901023,0.007851269],"study_design_scores_gemma":[0.0006675349,0.0006425674,0.001430866,0.00001449453,0.00006927495,0.00002074692,0.00008211446,0.00004559319,0.944408,0.0003254674,0.05218083,0.0001125218],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9876212,0.00841712,0.002669271,0.0003077021,0.0006031261,0.0001208044,0.000007236466,0.000004908173,0.0002485667],"genre_scores_gemma":[0.9961417,0.001520184,0.0009611091,0.0006306604,0.0001903174,0.000001900356,0.000008620764,0.00001227317,0.0005331762],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05199073,"threshold_uncertainty_score":0.4009202,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005183442002293678,"score_gpt":0.2626288147183143,"score_spread":0.2574453727160206,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}