{"id":"W4408938737","doi":"10.3390/bioengineering12040345","title":"Model-Based Characterization of the Metabolism of Recombinant Adeno-Associated Virus (rAAV) Production via Human Embryonic Kidney (HEK293) Cells","year":2025,"lang":"en","type":"article","venue":"Bioengineering","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; National Research Council Canada; University of Ottawa","funders":"National Research Council Canada","keywords":"Glutamine; Citric acid cycle; Glycolysis; Glutaminolysis; Pentose phosphate pathway; Metabolic pathway; Biochemistry; Biology; HEK 293 cells; Tricarboxylic acid; Metabolism; Metabolic engineering; Metabolite; Amino acid; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008799283,0.0001229144,0.0001537459,0.00006590551,0.00007100227,0.000006911612,0.0001434805,0.0001100192,0.00000523382],"category_scores_gemma":[0.00006472139,0.0001026982,0.0001282891,0.0001966543,0.00003423655,0.000005166153,0.00006401785,0.00006111773,4.047698e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001865393,"about_ca_system_score_gemma":0.00008422874,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001816116,"about_ca_topic_score_gemma":0.000003338995,"domain_scores_codex":[0.9993126,0.00003546659,0.000229562,0.000199102,0.00009010216,0.0001331267],"domain_scores_gemma":[0.9993936,0.000002371271,0.0001516715,0.0003119521,0.0001022704,0.00003809883],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001660012,0.0001218707,0.0002597867,0.0000904621,0.00004840491,5.836481e-8,0.00001095636,0.01543735,0.9832625,0.00003430625,0.0006069927,0.0001106756],"study_design_scores_gemma":[0.0002849862,0.00004420278,0.003598978,0.000109109,0.0000459013,2.030454e-7,0.000001991024,0.01274491,0.9821257,0.00003181052,0.0009124737,0.00009974829],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9922125,0.0001830928,0.006002144,0.00007511187,0.00122406,0.0002072167,0.00003527139,0.00001934682,0.00004127583],"genre_scores_gemma":[0.9990031,0.00004099704,0.00005562136,0.00006764323,0.0000522321,0.0000237967,0.00009092067,0.00001593715,0.0006497512],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00679062,"threshold_uncertainty_score":0.418791,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006399463272662952,"score_gpt":0.2240686346218941,"score_spread":0.2176691713492312,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}