{"id":"W4409128608","doi":"10.1016/j.jmb.2025.169120","title":"Deep Mutational Scanning of an Engineered High-affinity Ligand of the poly(A) Binding Protein MLLE Domain","year":2025,"lang":"en","type":"article","venue":"Journal of Molecular Biology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Fonds de Recherche du Québec - Santé; Canadian Institutes of Health Research","keywords":"Ligand (biochemistry); Chemistry; Computational biology; Plasma protein binding; Biophysics; Domain (mathematical analysis); Biology; Genetics; Cell biology; Biochemistry; Receptor; Mathematics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001654618,0.0003291483,0.0002173396,0.0000827571,0.0001145608,0.0001546302,0.0002222792,0.0002719505,0.001317323],"category_scores_gemma":[0.0001593176,0.0001167093,0.0002068025,0.00009275205,0.0001482051,0.0001230982,0.0002250075,0.0004735114,0.0003122447],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002544922,"about_ca_system_score_gemma":0.0001553326,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004409681,"about_ca_topic_score_gemma":0.0007388904,"domain_scores_codex":[0.9998807,0.00002325185,0.000007210758,0.00002852285,0.00003014327,0.00003023655],"domain_scores_gemma":[0.9998994,0.00002468425,0.00002115179,0.00001004293,0.00001654208,0.00002817818],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008088403,0.00002700883,0.0001153158,0.00001777656,0.000004535931,0.00005081486,0.000008379307,0.0003811089,0.9986598,0.00006302228,0.00002663505,0.0005647111],"study_design_scores_gemma":[0.00001217718,0.0001797873,0.0006040068,0.000002223541,0.000005908968,0.0001187005,0.0000101258,0.003628077,0.9946902,0.00001692505,0.0007265935,0.00000530145],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9928004,0.0001632391,0.005921921,0.00004818711,0.00001327838,0.00002557956,0.0001362954,0.00007125106,0.0008198416],"genre_scores_gemma":[0.9922571,0.00009103664,0.005905746,0.00005975244,0.000002757883,0.00002064323,0.0002940953,0.00002691816,0.001341907],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001317323,"threshold_uncertainty_score":0.004406869,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006500186723368244,"score_gpt":0.280849451416821,"score_spread":0.2743492646934528,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}