{"id":"W4409448893","doi":"10.2166/wh.2025.101","title":"Optimizing RT-qPCR multiplex assays for simultaneous detection of enteric and respiratory viruses in wastewater","year":2025,"lang":"en","type":"article","venue":"Journal of Water and Health","topic":"Respiratory viral infections research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ontario Tech University","funders":"","keywords":"Multiplex; Enterovirus; Virology; Biology; Outbreak; Microbiology; Coxsackievirus; Virus; Bioinformatics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001081703,0.00008172671,0.0003433163,0.0005605339,0.00007803465,0.00001952348,0.00003311242,0.00006065428,0.000004979215],"category_scores_gemma":[0.0001090192,0.00004827415,0.00004864431,0.0001038339,0.00005215763,0.0001088491,0.00003038756,0.0002303408,3.106126e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001220382,"about_ca_system_score_gemma":0.0001214256,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008901017,"about_ca_topic_score_gemma":0.00008809382,"domain_scores_codex":[0.9988891,0.00009363998,0.0005780477,0.0001077716,0.0001292764,0.0002021392],"domain_scores_gemma":[0.9994329,0.00009885951,0.0001181143,0.00007947444,0.0001680227,0.0001026839],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.002764323,0.0003083108,0.06373762,0.002437157,0.0001101769,0.00005532723,0.001980756,0.0004923372,0.8620115,0.00001227531,0.0001427994,0.06594741],"study_design_scores_gemma":[0.01588252,0.01438191,0.04794968,0.001712976,0.0001103102,0.0003334158,0.001259633,0.005977659,0.873861,0.0003720603,0.03789596,0.0002628271],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9959804,0.001094693,0.001851022,0.0005764613,0.0001048206,0.0003489172,0.000002201355,0.000004675031,0.0000367607],"genre_scores_gemma":[0.9980741,0.0001591627,0.0007758253,0.0008356176,0.00006869863,0.000006047367,3.878482e-7,0.000008891225,0.00007123736],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.06568457,"threshold_uncertainty_score":0.1968563,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08565578308775336,"score_gpt":0.4084199520229971,"score_spread":0.3227641689352437,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}