{"id":"W4409456893","doi":"10.17504/protocols.io.rm7vzkzzxvx1/v1","title":"Dengue Virus Serotype 2 NS2B-NS3 protease fusion protein Avi-tagged (Biotinylated) protein expression and purification: large scale 1 L cultures v1","year":2025,"lang":"en","type":"preprint","venue":"","topic":"Mosquito-borne diseases and control","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Discovery Centre","funders":"National Institute of Allergy and Infectious Diseases; National Institutes of Health","keywords":"NS3; Dengue virus; Protease; Biotinylation; Serotype; Virology; Dengue fever; Fusion protein; Virus; Biology; Chemistry; Molecular biology; Biochemistry; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005665479,0.001122306,0.001208047,0.0004135646,0.0006072475,0.0008798538,0.001103936,0.0005508502,0.009303809],"category_scores_gemma":[0.0005005699,0.0007736009,0.0005304461,0.0008123395,0.0003166599,0.0004034406,0.0008834099,0.001688551,0.01840485],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005835857,"about_ca_system_score_gemma":0.0006917313,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002410377,"about_ca_topic_score_gemma":0.002897917,"domain_scores_codex":[0.999437,0.00007841932,0.00003942426,0.0001159391,0.000232966,0.00009623404],"domain_scores_gemma":[0.9998206,0.00003608259,0.00001384808,0.00004972369,0.00005310199,0.00002663462],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009543483,0.00005161872,0.0001320802,0.0002160345,0.00001412459,0.00005328628,0.00006097202,0.0001451673,0.9853529,0.0004711811,0.007724547,0.005682648],"study_design_scores_gemma":[0.0000311557,0.0001096771,0.001433287,0.00003593493,0.00002275649,0.0002926002,0.00003576521,0.002173436,0.8956766,0.0004645664,0.09969433,0.00002996792],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.2872818,0.006922359,0.5401293,0.001723958,0.001444885,0.003900939,0.09888734,0.01619073,0.04351857],"genre_scores_gemma":[0.2583914,0.006360788,0.3549247,0.0006390524,0.0002415525,0.003294965,0.2624123,0.004329482,0.1094057],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.009303809,"threshold_uncertainty_score":0.03112435,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01068118032448357,"score_gpt":0.2767072245157133,"score_spread":0.2660260441912298,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}