{"id":"W4409558016","doi":"10.3390/microorganisms13040923","title":"Impact of Pre-Extraction Methods on Apple Blossom Microbiome Analysis","year":2025,"lang":"en","type":"article","venue":"Microorganisms","topic":"Plant Pathogens and Fungal Diseases","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Brock University; Agriculture and Agri-Food Canada","funders":"Agriculture and Agri-Food Canada","keywords":"Sonication; Biology; DNA extraction; Extraction (chemistry); Microbiome; Bacteria; Microbiology; Nucleic acid; Microbial population biology; Food science; Chromatography; Chemistry; Biochemistry; Polymerase chain reaction; Bioinformatics; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002746688,0.001003184,0.0008969615,0.0009271111,0.0006084137,0.001234115,0.0004547412,0.0005840581,0.0008546326],"category_scores_gemma":[0.004516198,0.000383817,0.000738677,0.0006712881,0.0005145548,0.0009343624,0.001216728,0.00102777,0.0006177545],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003285091,"about_ca_system_score_gemma":0.0006820426,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009420315,"about_ca_topic_score_gemma":0.003304446,"domain_scores_codex":[0.9973379,0.00070099,0.0003065142,0.0005483443,0.0009294299,0.0001768827],"domain_scores_gemma":[0.9978294,0.001149732,0.0002858658,0.0001745459,0.000462402,0.00009811898],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001922766,0.00007551008,0.004801082,0.0007504972,0.00007733578,0.00008106002,0.0001980413,0.0003482411,0.9727169,0.00007190992,0.0001736392,0.0205136],"study_design_scores_gemma":[0.00001065335,0.0007204515,0.04764694,0.0001452521,0.0001803891,0.0003387661,0.0002890612,0.002527495,0.9366242,0.0001602221,0.0112992,0.00005740471],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8991137,0.01452394,0.07973897,0.0005918807,0.0003852744,0.0007724091,0.001925739,0.0005834791,0.002364411],"genre_scores_gemma":[0.7958698,0.01236504,0.1788144,0.001085853,0.0001815927,0.001344541,0.006245932,0.0005583716,0.003534531],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002746688,"threshold_uncertainty_score":0.01452607,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006304064576850179,"score_gpt":0.3292025530323526,"score_spread":0.3228984884555025,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}