{"id":"W4409672987","doi":"10.1002/pro.70114","title":"Optimization of synthetic human <scp>V<sub>H</sub></scp> affinity and solubility through in vitro affinity maturation and minimal camelization","year":2025,"lang":"en","type":"article","venue":"Protein Science","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa; National Research Council Canada","funders":"Pacific Northwest National Laboratory; National Research Council Canada","keywords":"Thermostability; Affinity maturation; Phage display; Immunogenicity; Solubility; In vitro; Chemistry; Affinity chromatography; Antigen; Mutant; Biochemistry; Saturated mutagenesis; Mutagenesis; Stereochemistry; Combinatorial chemistry; Biology; Peptide; Enzyme; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006838992,0.0006486594,0.000423929,0.0002257487,0.0001969999,0.0005314844,0.0004967094,0.0004618365,0.0009417133],"category_scores_gemma":[0.0006593289,0.000252599,0.0003258012,0.0003109667,0.0002267178,0.0002747162,0.0002909032,0.0005081802,0.0004987395],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007808852,"about_ca_system_score_gemma":0.0003581391,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001196062,"about_ca_topic_score_gemma":0.001234693,"domain_scores_codex":[0.9995906,0.0001240202,0.00004830708,0.00006395847,0.0001096481,0.00006360155],"domain_scores_gemma":[0.9997616,0.00007256575,0.00005562982,0.0000180112,0.00004716714,0.00004494004],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001043507,0.00006462382,0.0001238639,0.0000430662,0.000009139159,0.00004333998,0.00002135452,0.0008516437,0.9973176,0.00009939542,0.00007000695,0.001251598],"study_design_scores_gemma":[0.00002194745,0.000412648,0.0004317204,0.000003759945,0.0000119658,0.00007386273,0.00001513412,0.001708952,0.9957877,0.00002135255,0.001503637,0.000007324136],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9824724,0.0006944729,0.01434921,0.0001037854,0.00002758323,0.0001895055,0.0004153827,0.0001309488,0.001616615],"genre_scores_gemma":[0.9817283,0.0005868076,0.01480822,0.00008801194,0.00001294361,0.0001070106,0.0009880558,0.00007334426,0.001607279],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001196062,"threshold_uncertainty_score":0.00566572,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02065155696037058,"score_gpt":0.3087178197718648,"score_spread":0.2880662628114942,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}