{"id":"W4409672987","doi":"10.1002/pro.70114","title":"Optimization of synthetic human <scp>V<sub>H</sub></scp> affinity and solubility through in vitro affinity maturation and minimal camelization","year":2025,"lang":"en","type":"article","venue":"Protein Science","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa; National Research Council Canada","funders":"Pacific Northwest National Laboratory; National Research Council Canada","keywords":"Thermostability; Affinity maturation; Phage display; Immunogenicity; Solubility; In vitro; Chemistry; Affinity chromatography; Antigen; Mutant; Biochemistry; Saturated mutagenesis; Mutagenesis; Stereochemistry; Combinatorial chemistry; Biology; Peptide; Enzyme; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009061235,0.0001106863,0.0002176635,0.0002004075,0.0002382285,0.00005009229,0.0001085369,0.00006890295,0.0000033773],"category_scores_gemma":[0.001336776,0.00009541439,0.00002272701,0.0007928073,0.0009174374,0.0003102831,0.0001677683,0.0001670366,9.364907e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005629251,"about_ca_system_score_gemma":0.0002179143,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001735901,"about_ca_topic_score_gemma":0.00003870569,"domain_scores_codex":[0.9985905,0.00008799901,0.0002909999,0.0003803897,0.0004056099,0.0002444797],"domain_scores_gemma":[0.9992843,0.0001429661,0.00009044151,0.0001769015,0.0002297866,0.00007566432],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001284708,0.0001275856,0.01085305,0.0003401995,0.000004213262,0.000002387724,0.0004002287,0.0001019387,0.9839171,0.001071644,0.000008532813,0.003044674],"study_design_scores_gemma":[0.0003723052,0.0001192676,0.1114697,0.0001562105,0.000009719747,0.000005628624,0.0001273595,0.009992588,0.8762584,0.001430602,0.00001912147,0.00003917533],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9954348,0.000227957,0.001770305,0.0005021496,0.00002860582,0.0007411481,0.000008790557,0.00001707925,0.001269123],"genre_scores_gemma":[0.9951476,0.00009258865,0.004571317,0.00004785926,0.00001550449,0.00003515864,0.00001017798,0.000004333184,0.0000754811],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1076587,"threshold_uncertainty_score":0.3890886,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02065155696037058,"score_gpt":0.3087178197718648,"score_spread":0.2880662628114942,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}