{"id":"W4409727299","doi":"10.17504/protocols.io.36wgqndzogk5/v1","title":"Enterovirus A71 3C protease small scale expression and purification protocol v1","year":2024,"lang":"en","type":"preprint","venue":"","topic":"Viral Infections and Immunology Research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Discovery Centre","funders":"National Institute of Allergy and Infectious Diseases; National Institutes of Health","keywords":"Scale (ratio); Protease; Protocol (science); Virology; Expression (computer science); Enterovirus; Enterovirus 71; Biology; Computer science; Virus; Geography; Enzyme; Medicine; Cartography; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001167452,0.002069349,0.001840759,0.00157312,0.001465696,0.001748576,0.002068218,0.0009835027,0.02656684],"category_scores_gemma":[0.001615367,0.001398991,0.001219676,0.001780112,0.0006190832,0.0007541893,0.001072594,0.004511865,0.04540775],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008765344,"about_ca_system_score_gemma":0.001333248,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002303143,"about_ca_topic_score_gemma":0.002608454,"domain_scores_codex":[0.9985365,0.0002144914,0.00014707,0.0002680675,0.0005428287,0.0002910575],"domain_scores_gemma":[0.9994146,0.0001244084,0.00003766163,0.00018324,0.0001509508,0.00008923817],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0006981368,0.0002434081,0.0003270149,0.001256783,0.0000699228,0.0002638431,0.0002335862,0.0004775231,0.9043007,0.004225672,0.06458684,0.02331654],"study_design_scores_gemma":[0.0001460318,0.0001970543,0.001362398,0.0001348566,0.00007189895,0.0006337467,0.00005788085,0.001403266,0.6495827,0.00163921,0.3446831,0.00008781438],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.07564215,0.01034381,0.5999272,0.002470264,0.004122637,0.01394652,0.2101666,0.02100356,0.0623772],"genre_scores_gemma":[0.08160647,0.009805826,0.2940285,0.001000719,0.0004779549,0.0135221,0.4794138,0.006333986,0.1138107],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.02656684,"threshold_uncertainty_score":0.08887494,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05206580666056466,"score_gpt":0.3660318857590497,"score_spread":0.313966079098485,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}