{"id":"W4409813124","doi":"10.17504/protocols.io.n92ld8rnov5b/v1","title":"Small-scale Expression and Purification Protocol for His-SUMO Tagged Enterovirus D68 Strain STL 2014 12 3C Protease in E. coli v1","year":2024,"lang":"en","type":"preprint","venue":"","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Discovery Centre","funders":"National Institute of Allergy and Infectious Diseases; National Institutes of Health","keywords":"Protease; Strain (injury); Chemistry; Scale (ratio); Protein expression; Molecular biology; Biology; Computational biology; Enzyme; Biochemistry; Physics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008110041,0.001215698,0.001381318,0.0009143044,0.0008752675,0.0008441166,0.001349476,0.0005553756,0.008122842],"category_scores_gemma":[0.0005723761,0.000953846,0.0008351257,0.001289017,0.0003674813,0.0004390655,0.0007007633,0.002554748,0.01466854],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006870492,"about_ca_system_score_gemma":0.0007263896,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001303657,"about_ca_topic_score_gemma":0.002498731,"domain_scores_codex":[0.9991854,0.0001228599,0.0001089974,0.0001494939,0.0003024521,0.0001308423],"domain_scores_gemma":[0.9997064,0.00005308504,0.00002999588,0.00008112392,0.00007818086,0.00005115075],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0001846532,0.0001153975,0.0001488131,0.0004659034,0.00002556593,0.0001963184,0.0001059095,0.0002629236,0.9853808,0.0007275403,0.00542146,0.006964643],"study_design_scores_gemma":[0.00008287266,0.0003242837,0.001800341,0.00008905806,0.00006505418,0.0009150624,0.00007331936,0.00128783,0.8107546,0.000616481,0.1839235,0.00006769425],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.2474859,0.01078509,0.5868049,0.001621513,0.001752017,0.01264858,0.09788058,0.008090707,0.03293064],"genre_scores_gemma":[0.1909122,0.009793425,0.4628893,0.0006601292,0.0002493192,0.01217587,0.2433056,0.002568082,0.07744608],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.008122842,"threshold_uncertainty_score":0.02717358,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08649365021834696,"score_gpt":0.3763317356653993,"score_spread":0.2898380854470524,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}