{"id":"W4410007660","doi":"10.1038/s41589-025-01898-0","title":"A heme-dependent enzyme forms the hydrazine in the antibiotic negamycin","year":2025,"lang":"en","type":"article","venue":"Nature Chemical Biology","topic":"Chemical Reactions and Isotopes","field":"Pharmacology, Toxicology and Pharmaceutics","cited_by":15,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia Hospital; University of British Columbia","funders":"Canadian Institutes of Health Research; Michael Smith Health Research BC; Government of Canada","keywords":"Heme; Chemistry; Enzyme; Antibiotics; Biochemistry; Hydrazine (antidepressant); Stereochemistry; Combinatorial chemistry; Microbiology; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["research_integrity"],"consensus_categories":[],"category_scores_codex":[0.0005989447,0.0002023476,0.0002532984,0.00007192692,0.0001351985,0.00001784295,0.0007167247,0.00114754,0.0002443659],"category_scores_gemma":[0.0005329894,0.0001008854,0.0001217305,0.0004857022,0.0003580581,0.0000362022,0.0001618109,0.003275051,0.00005763742],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00007851111,"about_ca_system_score_gemma":0.00005451906,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002047679,"about_ca_topic_score_gemma":0.00003374702,"domain_scores_codex":[0.9986121,0.0002074796,0.0003099973,0.0003403724,0.00008344092,0.000446595],"domain_scores_gemma":[0.9981138,0.001406543,0.00006529363,0.0003124968,0.00004528896,0.00005663973],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002248644,0.0003567564,0.004390383,0.00002445731,0.00008938905,0.00001278568,0.0001233187,0.000003929025,0.9347853,0.02783407,0.01779719,0.01435753],"study_design_scores_gemma":[0.0008837795,0.00003102271,0.0007234376,0.00001156484,0.00005359843,0.0000279653,0.00009236688,0.0001357996,0.5139825,0.006809203,0.4771237,0.0001250325],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9291757,0.004398546,0.00002631937,0.02914793,0.0008585957,0.0005209335,0.00003078865,0.00005949608,0.0357817],"genre_scores_gemma":[0.9797927,0.0003014564,0.00002581681,0.01907857,0.0002096236,0.00004009915,0.00004975846,0.00000777213,0.0004941788],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.4593265,"threshold_uncertainty_score":0.9990245,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0331340126702846,"score_gpt":0.4167656956796071,"score_spread":0.3836316830093225,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}