{"id":"W4410278214","doi":"10.1016/j.jmccpl.2025.100454","title":"Glyoxal is a superior fixative to formaldehyde in promoting antigenicity and structural integrity in murine cardiac tissues","year":2025,"lang":"en","type":"article","venue":"Journal of Molecular and Cellular Cardiology Plus","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Toronto General Hospital; University Health Network; Ted Rogers Centre for Heart Research; University of Toronto","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Heart and Stroke Foundation of Canada","keywords":"Fixative; Antigenicity; Glyoxal; Glutaraldehyde; Formaldehyde; Chemistry; Structural integrity; Cell biology; Biology; Biochemistry; Immunology; Chromatography; Antigen; Engineering; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006097787,0.0009024905,0.0002478311,0.0006514958,0.0002905304,0.0003078672,0.0002922411,0.0004909432,0.001636297],"category_scores_gemma":[0.0001861405,0.0002352504,0.0004205411,0.0002149503,0.0003847734,0.0003906551,0.0002094311,0.0005270176,0.0003170714],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003048946,"about_ca_system_score_gemma":0.0003258783,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005183005,"about_ca_topic_score_gemma":0.001207893,"domain_scores_codex":[0.9996482,0.00006876701,0.00003489568,0.0001136639,0.00007888779,0.00005556005],"domain_scores_gemma":[0.9995918,0.00004307849,0.0001928335,0.00004708327,0.00005224806,0.00007309639],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001701854,0.00002774484,0.0001748113,0.00006852731,0.000007138087,0.00004409102,0.000012973,0.00004200712,0.998553,0.0000632772,0.00002708291,0.0008091036],"study_design_scores_gemma":[0.00002498567,0.001096746,0.003863824,0.000028146,0.00005810141,0.0005005339,0.00002845568,0.000332162,0.9903765,0.00004410303,0.003635644,0.00001078911],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9653552,0.009549927,0.02037743,0.0003286173,0.000181695,0.0001718524,0.0006592117,0.0002958553,0.003080313],"genre_scores_gemma":[0.9398904,0.007072094,0.0452743,0.0002484771,0.00009913472,0.0003322181,0.00120939,0.0001696829,0.005704388],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001636297,"threshold_uncertainty_score":0.005473971,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005878166906422022,"score_gpt":0.2685975917063387,"score_spread":0.2627194247999167,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}