{"id":"W4410477065","doi":"10.1101/2025.05.14.653984","title":"R2HaPpY: Rapid-robust phosphotyrosine peptide enrichment using HaloTag-Src SH2 pY superbinder","year":2025,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Institute of General Medical Sciences; Canadian Institutes of Health Research; National Institutes of Health","keywords":"Proto-oncogene tyrosine-protein kinase Src; SH2 domain; Chemistry; Receptor; Biochemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001451916,0.001110731,0.0009053507,0.001107724,0.0005436474,0.0009022189,0.001506438,0.0009573878,0.005140684],"category_scores_gemma":[0.000868888,0.0008256256,0.0006014451,0.0005649248,0.0005886231,0.0006995738,0.001628548,0.001931262,0.004827882],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005965147,"about_ca_system_score_gemma":0.0007635691,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008807812,"about_ca_topic_score_gemma":0.002045943,"domain_scores_codex":[0.9985864,0.0001200149,0.00006161588,0.0003679945,0.0007009932,0.0001629013],"domain_scores_gemma":[0.999522,0.0001097698,0.00007701874,0.0001244489,0.00008268936,0.00008410911],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001270232,0.00002901178,0.0002129261,0.0001239443,0.0000270269,0.00005620225,0.00003384373,0.0002139634,0.9887775,0.0002366953,0.001885495,0.008276373],"study_design_scores_gemma":[0.00003763186,0.00006360448,0.001397685,0.00001052811,0.0000110893,0.0002754875,0.00001085637,0.004694017,0.9798813,0.0002042473,0.0133826,0.00003089832],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3044904,0.003095991,0.6539217,0.0008426781,0.000351403,0.001008118,0.008119928,0.0216458,0.006523989],"genre_scores_gemma":[0.3165099,0.003117101,0.626045,0.001099295,0.000155802,0.002896045,0.01754993,0.005989923,0.02663706],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.005140684,"threshold_uncertainty_score":0.01719731,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03823533494068081,"score_gpt":0.2798952511763119,"score_spread":0.2416599162356311,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}