{"id":"W4410487724","doi":"10.1016/j.omtn.2025.102571","title":"A novel miniaturized filamentous phagemid as a gene delivery vehicle to target mammalian cells","year":2025,"lang":"en","type":"article","venue":"Molecular Therapy — Nucleic Acids","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada; Mitacs; Consejo Nacional de Ciencia y Tecnología","keywords":"Phagemid; Gene delivery; Transgene; Biology; DNA; Plasmid; Phage display; Gene; In vitro recombination; Molecular biology; Computational biology; Cell biology; Genetic enhancement; Bacteriophage; Gene expression; Genetics; Molecular cloning; Escherichia coli","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000208953,0.0002954745,0.0001694209,0.0001798916,0.0001571141,0.0002662397,0.0003711872,0.0003158233,0.0006563384],"category_scores_gemma":[0.0001038382,0.0001230103,0.0001863179,0.0001316674,0.0001536122,0.0002065032,0.0002492724,0.0003510462,0.0003735591],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00035112,"about_ca_system_score_gemma":0.0003278083,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000462468,"about_ca_topic_score_gemma":0.0007197884,"domain_scores_codex":[0.9999101,0.00001115963,0.000005836036,0.0000199835,0.00004011162,0.00001272225],"domain_scores_gemma":[0.9999511,0.000007632532,0.00001160399,0.000004422581,0.000007572752,0.00001767231],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001854309,0.00001380693,0.00009897096,0.00003387758,0.000002647135,0.00004423419,0.000008619694,0.00009620967,0.9955714,0.0003456256,0.00009231532,0.003673854],"study_design_scores_gemma":[0.0000121888,0.0003185964,0.0009936879,0.000005546541,0.00001267186,0.0004386918,0.000007722621,0.001502062,0.9840045,0.00008911672,0.01260535,0.0000099119],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7590061,0.003094437,0.2299634,0.0002922927,0.0001980961,0.000395809,0.0008664645,0.00153205,0.004651411],"genre_scores_gemma":[0.8181267,0.002022886,0.1649196,0.000126406,0.00004082135,0.0002259917,0.00131683,0.000116237,0.0131044],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006563384,"threshold_uncertainty_score":0.002547562,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006831916206813515,"score_gpt":0.2389225969171793,"score_spread":0.2320906807103658,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}