{"id":"W4410546428","doi":"10.1038/s41587-025-02649-1","title":"A multi-kingdom genetic barcoding system for precise clone isolation","year":2025,"lang":"en","type":"article","venue":"Nature Biotechnology","topic":"Genomics and Phylogenetic Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"BC Children's Hospital; University of British Columbia","funders":"Canadian Institutes of Health Research; Japan Science and Technology Agency; Japan Student Services Organization; Secom Science and Technology Foundation; Japan Society for the Promotion of Science; Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada; New Energy and Industrial Technology Development Organization; Ministry of Education, Culture, Sports, Science and Technology; Takeda Science Foundation; Allen Foundation; Michael Smith Health Research BC; Japan Society for the Promotion of Science London; Japan Agency for Medical Research and Development; Canada Foundation for Innovation","keywords":"clone (Java method); Isolation (microbiology); DNA barcoding; Biology; Evolutionary biology; Computational biology; Genetics; Gene; Bioinformatics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001546139,0.0007225895,0.0009789165,0.001212752,0.0006884919,0.001120065,0.001065387,0.001443333,0.003787411],"category_scores_gemma":[0.001953537,0.0009713308,0.0007546699,0.0007372185,0.0007471307,0.0009738717,0.001823456,0.002101625,0.005877225],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005075263,"about_ca_system_score_gemma":0.0006382287,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003760403,"about_ca_topic_score_gemma":0.001052827,"domain_scores_codex":[0.9981776,0.0002611717,0.0001755318,0.0005349222,0.0006932374,0.0001575597],"domain_scores_gemma":[0.9976293,0.0005144941,0.0005546614,0.0007476263,0.0003311471,0.0002227722],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003195282,0.00001535658,0.000131209,0.00007582093,0.000007537548,0.00004642423,0.00004614975,0.00008943478,0.9903865,0.0005641447,0.000552302,0.008053155],"study_design_scores_gemma":[0.00001314495,0.0001187578,0.0009512579,0.00002285579,0.0000235339,0.0005273089,0.00001665407,0.002350871,0.9688292,0.0002107747,0.02690174,0.00003387399],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.06899429,0.001110017,0.9093171,0.0003946685,0.0002687493,0.0007198693,0.002903484,0.01230807,0.003983779],"genre_scores_gemma":[0.2310877,0.0017683,0.7395839,0.0006985534,0.00008108065,0.0016439,0.01077484,0.002778859,0.01158281],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.003787411,"threshold_uncertainty_score":0.0126701,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008549519772962205,"score_gpt":0.2603567706534956,"score_spread":0.2518072508805334,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}