{"id":"W4410546428","doi":"10.1038/s41587-025-02649-1","title":"A multi-kingdom genetic barcoding system for precise clone isolation","year":2025,"lang":"en","type":"article","venue":"Nature Biotechnology","topic":"Genomics and Phylogenetic Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"BC Children's Hospital; University of British Columbia","funders":"Canadian Institutes of Health Research; Japan Science and Technology Agency; Japan Student Services Organization; Secom Science and Technology Foundation; Japan Society for the Promotion of Science; Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada; New Energy and Industrial Technology Development Organization; Ministry of Education, Culture, Sports, Science and Technology; Takeda Science Foundation; Allen Foundation; Michael Smith Health Research BC; Japan Society for the Promotion of Science London; Japan Agency for Medical Research and Development; Canada Foundation for Innovation","keywords":"clone (Java method); Isolation (microbiology); DNA barcoding; Biology; Evolutionary biology; Computational biology; Genetics; Gene; Bioinformatics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001141042,0.0001724206,0.0001965893,0.0001387442,0.0001528238,0.00001482372,0.0002613257,0.001171071,7.277169e-7],"category_scores_gemma":[0.0001580232,0.000166743,0.00009660793,0.0001600151,0.00008011854,4.722686e-7,0.0001853508,0.0002146315,0.000002106443],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003485857,"about_ca_system_score_gemma":0.00005092941,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007919155,"about_ca_topic_score_gemma":0.00002568644,"domain_scores_codex":[0.9989767,0.00002149756,0.0002144552,0.0004731208,0.00004715095,0.0002670401],"domain_scores_gemma":[0.9993678,0.00002270183,0.00008034467,0.0004017581,0.0001029782,0.00002444638],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000714686,0.0000270693,0.001098701,0.00007079886,0.0001048701,8.97153e-7,0.00001050382,0.00004069702,0.9907162,0.002346479,0.0006651471,0.004847163],"study_design_scores_gemma":[0.001542198,0.0002435182,0.005950628,0.0000532071,0.00007758813,0.00001670005,0.0001216057,0.001581745,0.8628523,0.0003981053,0.1268719,0.0002905611],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8971617,0.01668568,0.08291451,0.001122219,0.001049433,0.0007750025,0.0000563329,0.00005365556,0.000181465],"genre_scores_gemma":[0.9592751,0.0002938538,0.03981712,0.0001622895,0.0001375574,0.0001019387,0.00002578227,0.00001844328,0.0001679193],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.127864,"threshold_uncertainty_score":0.9032369,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008549519772962205,"score_gpt":0.2603567706534956,"score_spread":0.2518072508805334,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}