{"id":"W4410565628","doi":"10.17504/protocols.io.q26g7186qgwz/v2","title":"Fluorescence assay for Enterovirus coxsackievirus A16 2A protease activity measurement v2","year":2024,"lang":"en","type":"preprint","venue":"","topic":"Viral Infections and Immunology Research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Discovery Centre","funders":"National Institute of Allergy and Infectious Diseases; National Institutes of Health","keywords":"Coxsackievirus; Virology; Enterovirus 71; Enterovirus; Fluorescence; Protease; Microbiology; Chemistry; Biology; Enzyme; Virus; Biochemistry; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001345665,0.002189066,0.001338418,0.001435532,0.0007946498,0.0009909642,0.00111388,0.001162507,0.01476318],"category_scores_gemma":[0.001106978,0.0007456384,0.00104245,0.001100418,0.0003747527,0.0006043501,0.0007448281,0.00252713,0.01669956],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006967023,"about_ca_system_score_gemma":0.0005060517,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006964646,"about_ca_topic_score_gemma":0.0007005889,"domain_scores_codex":[0.9980541,0.0003858124,0.0001490404,0.0004844324,0.0007084998,0.0002180803],"domain_scores_gemma":[0.9993328,0.0001558868,0.00004977029,0.0001628017,0.0002273546,0.00007131576],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001682817,0.00009816833,0.0002258551,0.0002291265,0.00002115857,0.00004560387,0.0000614184,0.0001259054,0.9889019,0.0006087802,0.002041252,0.007472802],"study_design_scores_gemma":[0.00002876955,0.0002737916,0.001331981,0.00006444286,0.00003644001,0.0003591644,0.00002484875,0.001084209,0.9605302,0.0002666002,0.0359645,0.0000350611],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2354459,0.01055826,0.6686221,0.0007749005,0.001445601,0.004956382,0.0252795,0.009996014,0.04292143],"genre_scores_gemma":[0.2457819,0.01002518,0.5146478,0.00049535,0.0003025299,0.008833462,0.09558149,0.003309624,0.1210227],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01476318,"threshold_uncertainty_score":0.04938775,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1140311132406151,"score_gpt":0.3740807990271983,"score_spread":0.2600496857865832,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}