{"id":"W4410586666","doi":"10.1002/pro.70160","title":"The enterobactin biosynthetic intermediate 2,3‐dihydroxybenzoic acid is a competitive inhibitor of the <i>Escherichia coli</i> isochorismatase <scp>EntB</scp>","year":2025,"lang":"en","type":"article","venue":"Protein Science","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University","funders":"Natural Sciences and Engineering Research Council of Canada; McMaster University","keywords":"Enterobactin; Chemistry; Escherichia coli; Stereochemistry; Phosphofructokinase 2; Enzyme kinetics; Substrate (aquarium); Isothermal titration calorimetry; Enzyme; Active site; Biochemistry; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001208871,0.0002090876,0.000210099,0.0000975505,0.000902207,0.0002663471,0.001421679,0.00006869495,0.00004593116],"category_scores_gemma":[0.0009518164,0.0001186685,0.0001065163,0.001275119,0.002055758,0.0003368072,0.000521603,0.0002443947,0.00006833488],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001033329,"about_ca_system_score_gemma":0.0003159908,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005579516,"about_ca_topic_score_gemma":0.00003767591,"domain_scores_codex":[0.9977196,0.0001775375,0.0004156175,0.0005215925,0.0006662667,0.0004994166],"domain_scores_gemma":[0.9984121,0.0002091187,0.0003266495,0.0007373121,0.0002287456,0.0000861126],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001452775,0.00003106596,0.0001940759,0.00002924045,0.000005266937,5.601497e-7,0.0007385369,0.000002086607,0.9943413,0.002907296,0.001232449,0.0005035848],"study_design_scores_gemma":[0.0002217088,0.00007067607,0.001281383,0.0001919723,0.00001731579,0.000001887472,0.0006909465,0.0001048524,0.9842965,0.001541871,0.01152201,0.0000588687],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9921052,0.0002896059,0.0004817764,0.0009391654,0.00182642,0.0008580835,0.00006282708,0.00004902873,0.003387882],"genre_scores_gemma":[0.9979628,0.00001670937,0.0003176735,0.0007011567,0.00007814053,0.000106159,9.428065e-7,0.00000926704,0.0008071408],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01028956,"threshold_uncertainty_score":0.7574525,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005129475096218312,"score_gpt":0.2235586538958741,"score_spread":0.2184291787996558,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}