{"id":"W4410823796","doi":"10.1128/mbio.00382-25","title":"CRISPR/dCas-mediated counter-silencing: reprogramming dCas proteins into antagonists of xenogeneic silencers","year":2025,"lang":"en","type":"article","venue":"mBio","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Canadian Institutes of Health Research; Deutsche Forschungsgemeinschaft; Jürgen Manchot Stiftung","keywords":"Biology; Gene silencing; Nucleoid; Genetics; RNA interference; CRISPR; CRISPR interference; Promoter; Computational biology; RNA; Gene; Cas9; Cell biology; Gene expression; Escherichia coli","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001392809,0.0004486187,0.0002924393,0.0002020131,0.0001404782,0.0004325063,0.0002728959,0.0002251543,0.0009482517],"category_scores_gemma":[0.0001123033,0.0001493602,0.0002355294,0.0001617153,0.0002772773,0.0001375932,0.0003480272,0.0006256245,0.0003606779],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004229481,"about_ca_system_score_gemma":0.0002469367,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001021669,"about_ca_topic_score_gemma":0.001623328,"domain_scores_codex":[0.9998129,0.00001724176,0.00001342779,0.0000676749,0.00006001586,0.00002866908],"domain_scores_gemma":[0.9998821,0.00001529734,0.00004223418,0.00001962964,0.00001550102,0.0000252703],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001457364,0.00000412789,0.00007484948,0.00001483805,0.00000206357,0.000015428,0.000004408492,0.00006332922,0.9989831,0.00006507868,0.0000139654,0.0007442628],"study_design_scores_gemma":[0.000005130803,0.00006013251,0.0008701021,0.000003317042,0.00000831978,0.0001243944,0.000012636,0.001200822,0.9942014,0.00002315173,0.003486089,0.000004641988],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9583287,0.001489518,0.03502576,0.0001560567,0.0001014136,0.0001115165,0.0007851289,0.0006479087,0.003354032],"genre_scores_gemma":[0.9828797,0.0005922668,0.01107294,0.00007027941,0.00001059572,0.00005073132,0.0006289982,0.00008668473,0.004607881],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001021669,"threshold_uncertainty_score":0.003172219,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004913511512568206,"score_gpt":0.3058854869972209,"score_spread":0.3009719754846527,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}