{"id":"W4410904927","doi":"10.1101/2025.05.29.656827","title":"Proximity biotinylation at the host- <i>Shigella</i> interface reveals UFMylation as an antibacterial pathway","year":2025,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Dalhousie University","funders":"National Institute of General Medical Sciences; Dalhousie University; Horizon 2020 Framework Programme; Fonds Wetenschappelijk Onderzoek; Vlaamse regering; Universiteit Gent; European Commission; Wellcome Trust; National Science Foundation; London School of Hygiene and Tropical Medicine; Centro Nacional de Biotecnología; Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung","keywords":"Shigella; Biotinylation; Intracellular parasite; Intracellular; Biology; Cell biology; Ubiquitin ligase; Bacteria; Effector; Autophagy; Microbiology; DNA ligase; Salmonella; Ubiquitin; Biochemistry; Genetics; DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001244618,0.0002048928,0.0001291814,0.0001123941,0.0001393178,0.0002352888,0.0001638931,0.0002776156,0.001922379],"category_scores_gemma":[0.0001405564,0.0001211059,0.0001373667,0.00008126668,0.0002151026,0.0003016533,0.0003017601,0.0003446859,0.0006459327],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000309101,"about_ca_system_score_gemma":0.0001206105,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006372465,"about_ca_topic_score_gemma":0.0007455947,"domain_scores_codex":[0.9999288,0.000009387929,0.000002920499,0.00002286277,0.00001663299,0.00001924706],"domain_scores_gemma":[0.9999492,0.00001130474,0.00001437699,0.000007617254,0.000007283937,0.00001021841],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003663859,0.000002462602,0.00020745,0.00001719199,0.00000157908,0.00002407531,0.000008770036,0.00003845822,0.9989337,0.0001926931,0.00003448533,0.0005025119],"study_design_scores_gemma":[0.000002502622,0.00001893377,0.001774378,0.000002636873,0.000001892674,0.0001199807,0.0000138586,0.0006566,0.9960757,0.0001061938,0.001225067,0.00000229881],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9760092,0.0008998695,0.01884256,0.0002263261,0.00003149299,0.00001306406,0.0003207036,0.000168307,0.00348838],"genre_scores_gemma":[0.9898128,0.0003378987,0.006541875,0.00006211788,0.000005707925,0.00001076665,0.0002800023,0.00005247598,0.002896428],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001922379,"threshold_uncertainty_score":0.006430984,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009259884667045134,"score_gpt":0.2338561285839634,"score_spread":0.2245962439169183,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}