{"id":"W4410989195","doi":"10.1016/j.pep.2025.106752","title":"Strategies for generating soluble and monomeric samples of Ycf1p NBD2","year":2025,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Peptidase Inhibition and Analysis","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Monomer; Chemistry; Computational biology; Biophysics; Biochemistry; Biology; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008707083,0.001230187,0.0006593532,0.0006012669,0.0006673692,0.0009284141,0.0009150713,0.000606981,0.001330788],"category_scores_gemma":[0.0008015766,0.0005301241,0.0004144994,0.0007972723,0.0004295537,0.0004473568,0.0005152925,0.002189346,0.001222228],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001193836,"about_ca_system_score_gemma":0.0008440313,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00332408,"about_ca_topic_score_gemma":0.006422477,"domain_scores_codex":[0.9994146,0.00006960615,0.00007038723,0.0001235346,0.0002152935,0.0001065752],"domain_scores_gemma":[0.9996611,0.00007174583,0.00006177085,0.00007060899,0.00008139983,0.00005339574],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000634012,0.00003468229,0.00012256,0.00006917839,0.00001179688,0.00005651487,0.00005287208,0.0001179989,0.996994,0.0003643834,0.0003425358,0.00177014],"study_design_scores_gemma":[0.00001514743,0.00002617791,0.0006370457,0.000007161038,0.00001261419,0.0001187539,0.00003055016,0.001211082,0.9883625,0.0001143995,0.009454091,0.00001038415],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.5575052,0.004509152,0.413063,0.001831269,0.0004407871,0.001671518,0.0107126,0.002378086,0.007888358],"genre_scores_gemma":[0.6171443,0.00505988,0.3072805,0.0007141368,0.000100241,0.002590799,0.0432141,0.001176686,0.02271926],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.00332408,"threshold_uncertainty_score":0.008661926,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02856417932052471,"score_gpt":0.2994392491202305,"score_spread":0.2708750697997058,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}