{"id":"W4411021338","doi":"10.17504/protocols.io.14egn6zjql5d/v3","title":"Fluorescence assay for Enterovirus A71 3C protease activity measurement for screening against antiviral molecules v2","year":2024,"lang":"en","type":"preprint","venue":"","topic":"Viral Infections and Immunology Research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Discovery Centre","funders":"National Institute of Allergy and Infectious Diseases; National Institutes of Health","keywords":"Fluorescence; Virology; Enterovirus; Protease; Chemistry; Enterovirus 71; Biochemistry; Biology; Virus; Enzyme; Physics; Optics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009157638,0.00203381,0.001336721,0.00136437,0.0006184136,0.0006735813,0.0009047959,0.001194899,0.009404999],"category_scores_gemma":[0.0006466425,0.0005132344,0.001003789,0.001028835,0.0002960689,0.0004735423,0.0005992631,0.002251753,0.008845495],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006114143,"about_ca_system_score_gemma":0.000361877,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004944338,"about_ca_topic_score_gemma":0.0005332774,"domain_scores_codex":[0.998422,0.000356192,0.0001112632,0.0003164212,0.0005874389,0.0002066323],"domain_scores_gemma":[0.9995653,0.0001071935,0.00003686893,0.00008518765,0.0001503398,0.00005516255],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001428933,0.0001046791,0.0001503438,0.0001889858,0.00001946481,0.00003753694,0.00003644129,0.0001369315,0.9923794,0.0003846113,0.001284447,0.005134167],"study_design_scores_gemma":[0.00002418956,0.0003259278,0.00105309,0.00004294518,0.00003716,0.0002986495,0.00001928582,0.001090168,0.9806468,0.0001927226,0.01624206,0.00002703161],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3140981,0.01154668,0.6013491,0.000804441,0.000926049,0.003794296,0.01999047,0.007733187,0.03975774],"genre_scores_gemma":[0.3747021,0.0106356,0.4535638,0.0004767487,0.0002426226,0.007090581,0.06646677,0.001784983,0.08503684],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.009404999,"threshold_uncertainty_score":0.03146279,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1008549280105265,"score_gpt":0.3637299783967682,"score_spread":0.2628750503862417,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}