{"id":"W4411148571","doi":"10.1080/19336950.2025.2513268","title":"Structural and functional mapping of ion access pathways in the human K <sup>+</sup> -dependent Na <sup>+</sup> /Ca <sup>2+</sup> exchanger NCKX2 using cysteine scanning mutagenesis, thiol-modifying reagents, and homology modelling","year":2025,"lang":"en","type":"article","venue":"Channels","topic":"Ion channel regulation and function","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Canadian Institutes of Health Research; Alliance de recherche numérique du Canada; Canada Research Chairs","keywords":"Cysteine; Chemistry; Thiol; Mutagenesis; Ion; Reagent; Ion exchange; Radiochemistry; Biochemistry; Mutation; Enzyme; Physical chemistry; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0008099116,0.0004318501,0.0004580125,0.0004685783,0.0005843123,0.0001459766,0.0002898304,0.0004354053,0.00003886468],"category_scores_gemma":[0.0000672337,0.0003839954,0.0001339567,0.0003889106,0.0001856752,0.00007470937,0.0003934053,0.0003016338,0.000001277867],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00008197645,"about_ca_system_score_gemma":0.00009467139,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002318068,"about_ca_topic_score_gemma":0.000007075536,"domain_scores_codex":[0.9973053,0.000310546,0.000675836,0.0008409675,0.0003401481,0.0005272154],"domain_scores_gemma":[0.9989495,0.00007029732,0.0002476777,0.0004150729,0.000215504,0.0001019946],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"simulation_or_modeling","study_design_gemma":"simulation_or_modeling","study_design_scores_codex":[0.000163679,0.00005117123,0.00165411,0.0002968893,0.0001292697,0.00001298785,0.004115527,0.9497989,0.0417467,0.0002982766,0.000133327,0.001599119],"study_design_scores_gemma":[0.001888204,0.0001655279,0.0008914446,0.000281642,0.00007848658,0.0001127826,0.006152139,0.9740424,0.01481256,0.0006695979,0.0004516729,0.0004535098],"study_design_candidate":"simulation_or_modeling","study_design_consensus":"simulation_or_modeling","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9590994,0.001851698,0.03771918,0.0001986387,0.0003255802,0.0005723735,0.00004423383,0.00002872127,0.0001601456],"genre_scores_gemma":[0.9975042,0.0001962626,0.0002479733,0.0003507006,0.0009466634,0.0000609857,0.0003868378,0.00004344157,0.0002629391],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03840477,"threshold_uncertainty_score":0.9998612,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06472067203565243,"score_gpt":0.2846655253045391,"score_spread":0.2199448532688867,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}