{"id":"W4411148571","doi":"10.1080/19336950.2025.2513268","title":"Structural and functional mapping of ion access pathways in the human K <sup>+</sup> -dependent Na <sup>+</sup> /Ca <sup>2+</sup> exchanger NCKX2 using cysteine scanning mutagenesis, thiol-modifying reagents, and homology modelling","year":2025,"lang":"en","type":"article","venue":"Channels","topic":"Ion channel regulation and function","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Canadian Institutes of Health Research; Alliance de recherche numérique du Canada; Canada Research Chairs","keywords":"Cysteine; Chemistry; Thiol; Mutagenesis; Ion; Reagent; Ion exchange; Radiochemistry; Biochemistry; Mutation; Enzyme; Physical chemistry; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001099731,0.0002529257,0.0001793856,0.0001052327,0.0001713288,0.0001682486,0.0002835766,0.0002885344,0.0007827127],"category_scores_gemma":[0.0001530477,0.0001781563,0.0002814155,0.0001564238,0.0001559951,0.0001879848,0.0001879924,0.000236168,0.0001279358],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003583745,"about_ca_system_score_gemma":0.0003043938,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003342373,"about_ca_topic_score_gemma":0.003549025,"domain_scores_codex":[0.9999559,0.000007107209,0.000003298244,0.00001310526,0.00001305926,0.000007490261],"domain_scores_gemma":[0.9999397,0.00001652934,0.00001758516,0.000006529739,0.000008730976,0.00001088797],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006727292,0.0001037839,0.007597717,0.0002010816,0.0000531327,0.0005108362,0.0001902593,0.0371479,0.9465238,0.001114521,0.000224394,0.005659783],"study_design_scores_gemma":[0.0001231129,0.0006808723,0.05907905,0.00003112818,0.00009824765,0.0007275432,0.0003184478,0.4776488,0.4570757,0.0006547386,0.003498324,0.0000641786],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9975909,0.0001311904,0.001777597,0.0000248345,0.00000191034,0.000006789519,0.0001228978,0.00003730128,0.0003065721],"genre_scores_gemma":[0.9958503,0.0001244395,0.003494415,0.000009709557,9.477235e-7,0.00001151523,0.0002950587,0.00001253342,0.0002009816],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003342373,"threshold_uncertainty_score":0.006645799,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06472067203565243,"score_gpt":0.2846655253045391,"score_spread":0.2199448532688867,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}