{"id":"W4411191557","doi":"10.1016/j.jbc.2025.110357","title":"Structural basis for the substrate specificity of Helix pomatia AMP deaminase and a chimeric ADGF adenosine deaminase","year":2025,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Biochemical and Molecular Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Brookhaven National Laboratory; Argonne National Laboratory; Basic Energy Sciences; National Center for Research Resources; National Institute of General Medical Sciences; Office of Science; National Institutes of Health; Cancer Prevention and Research Institute of Texas; New England Biolabs; York University; U.S. Department of Energy","keywords":"Helix pomatia; Adenosine deaminase; AMP deaminase; Chemistry; Substrate specificity; Biochemistry; Adenosine; Substrate (aquarium); Enzyme; Biology; Snail","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002933998,0.0001663057,0.0002743377,0.00002239822,0.00008303904,0.00002202076,0.0003356278,0.0002363012,0.00004779905],"category_scores_gemma":[0.0005968685,0.00009668209,0.0002192407,0.0001250212,0.0003615831,0.000003241452,0.0001543788,0.0002156258,3.332534e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001381488,"about_ca_system_score_gemma":0.0000842923,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00000515715,"about_ca_topic_score_gemma":0.00000177634,"domain_scores_codex":[0.9989663,0.00004333508,0.0004101729,0.0002215512,0.0001305462,0.0002280667],"domain_scores_gemma":[0.9990861,0.0001228158,0.0002273043,0.0002159607,0.0002411654,0.0001066642],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005893729,0.00006252772,0.001762232,0.00009794814,0.0001232483,0.00000475166,0.000005958328,0.000003638327,0.994023,0.00001949459,0.0004828611,0.002824948],"study_design_scores_gemma":[0.0005994922,0.0002496646,0.008376241,0.00004635894,0.00004582845,0.00008768341,0.00008048821,0.00002888967,0.9869682,0.0001873036,0.003218381,0.0001115032],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9908469,0.007155305,0.001195474,0.0004278935,0.00004547153,0.0001381996,0.00004731707,0.000002177731,0.0001412144],"genre_scores_gemma":[0.9977288,0.0008024648,0.0009295064,0.0001012573,0.0001664789,0.00000801536,0.00002953937,0.000006788805,0.0002271117],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007054853,"threshold_uncertainty_score":0.3942581,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02425743944456749,"score_gpt":0.2903980490248446,"score_spread":0.2661406095802771,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}