{"id":"W4411255095","doi":"10.1021/acs.bioconjchem.5c00090","title":"Simple Diaminonucleoside-Mediated Nonenzymatic Ligation of Oligonucleotides","year":2025,"lang":"en","type":"article","venue":"Bioconjugate Chemistry","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada; Human Frontier Science Program","keywords":"Oligonucleotide; Chemistry; Ligation; Chemical ligation; Combinatorial chemistry; Reagent; Nucleic acid; DNA; Chemical biology; Covalent bond; RNA; Native chemical ligation; Chemical synthesis; Biochemistry; Organic chemistry; Amino acid; Molecular biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005091119,0.0007077681,0.0004128973,0.0002799522,0.0002527134,0.0003381093,0.0006108889,0.0005202204,0.001131259],"category_scores_gemma":[0.0004415296,0.0003314003,0.0002788135,0.0003168544,0.0003618381,0.0003140347,0.0004419454,0.0006739364,0.001249145],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002989757,"about_ca_system_score_gemma":0.000301007,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001840048,"about_ca_topic_score_gemma":0.0005151775,"domain_scores_codex":[0.9993827,0.0001317782,0.00005795334,0.0002052253,0.0001534423,0.00006889538],"domain_scores_gemma":[0.9998255,0.00003217204,0.00004614369,0.00003484317,0.00002910901,0.00003229118],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002117714,0.00001040921,0.00004077009,0.00007442384,0.000004916069,0.00005533844,0.00002166052,0.0001185909,0.9969618,0.0003040803,0.0000479487,0.00233892],"study_design_scores_gemma":[0.000004027995,0.00007449273,0.00007814703,0.000002705534,0.000004877701,0.0001062476,0.000003465748,0.0004410809,0.9958054,0.00005011417,0.003423531,0.000005895498],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.640619,0.006242843,0.3416775,0.0002752805,0.0002098598,0.0006118588,0.0007320812,0.00188912,0.007742369],"genre_scores_gemma":[0.8113511,0.003195949,0.1748098,0.0001717494,0.00004420807,0.0003841576,0.001091008,0.000196392,0.008755728],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001131259,"threshold_uncertainty_score":0.003784478,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005590166374438341,"score_gpt":0.2541967724531897,"score_spread":0.2486066060787514,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}