{"id":"W4411255095","doi":"10.1021/acs.bioconjchem.5c00090","title":"Simple Diaminonucleoside-Mediated Nonenzymatic Ligation of Oligonucleotides","year":2025,"lang":"en","type":"article","venue":"Bioconjugate Chemistry","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada; Human Frontier Science Program","keywords":"Oligonucleotide; Chemistry; Ligation; Chemical ligation; Combinatorial chemistry; Reagent; Nucleic acid; DNA; Chemical biology; Covalent bond; RNA; Native chemical ligation; Chemical synthesis; Biochemistry; Organic chemistry; Amino acid; Molecular biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000139678,0.0001929552,0.0002398439,0.00004628107,0.00006328546,0.00001728719,0.000200042,0.0002165971,0.000009302785],"category_scores_gemma":[0.0002100637,0.0001827928,0.000140949,0.0002401816,0.0001906044,0.000004471152,0.0001154536,0.00007845196,0.000003468575],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001918764,"about_ca_system_score_gemma":0.00006761964,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001541166,"about_ca_topic_score_gemma":0.000004006522,"domain_scores_codex":[0.9989273,0.00002308892,0.0003838602,0.0003497826,0.0001088902,0.0002070647],"domain_scores_gemma":[0.9991184,0.00002403811,0.000182368,0.0004656541,0.000153075,0.00005648793],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003521605,0.00005844683,0.001612558,0.0001424578,0.00008147694,0.000001191767,0.000005493691,0.000003535268,0.9955872,0.00002817173,0.001535775,0.0009085159],"study_design_scores_gemma":[0.0002654955,0.00003427998,0.001045305,0.00006631883,0.00004700476,0.000002474788,0.00005829444,0.0000805917,0.9920843,0.0003365109,0.005799072,0.0001804166],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9946218,0.0003215246,0.0009574346,0.0001143359,0.00003159328,0.0001467917,0.00004697639,0.00006456736,0.003694957],"genre_scores_gemma":[0.9969823,0.0001977364,0.001406843,0.0001953969,0.00006693397,0.00001310613,0.0003818805,0.00001624103,0.0007395746],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004263298,"threshold_uncertainty_score":0.7454076,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005590166374438341,"score_gpt":0.2541967724531897,"score_spread":0.2486066060787514,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}