{"id":"W4411264601","doi":"10.3791/68005","title":"An Electroporation Cytometry Protocol for Live-Cell, Fluorescence Microscopy Using U2 OS Cell Culture","year":2025,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Microbial Inactivation Methods","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Toronto Metropolitan University; St. Michael's Hospital","funders":"","keywords":"Electroporation; Fluorescence microscope; Flow cytometry; Cytometry; Live cell imaging; Microscopy; Cell biology; Cell; Cell culture; Chemistry; Molecular biology; Fluorescence; Biology; Pathology; Medicine; Physics; Genetics; Biochemistry; Optics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003876489,0.0001719458,0.000226786,0.000133701,0.0001235912,0.00008796201,0.0002438694,0.0001850875,0.00003387395],"category_scores_gemma":[0.00007206992,0.0001535098,0.0001305244,0.0001742124,0.00003511158,0.00002848082,0.00003644735,0.0001215506,9.539317e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00009764289,"about_ca_system_score_gemma":0.0002563598,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00000529886,"about_ca_topic_score_gemma":1.892189e-7,"domain_scores_codex":[0.9987349,0.000219376,0.0004845845,0.0002226178,0.0001398648,0.0001986736],"domain_scores_gemma":[0.9987503,0.00001007931,0.0005397157,0.0001960705,0.0004285891,0.00007523687],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001234801,0.0005115611,0.0001410997,0.00006937729,0.0000345123,0.000001022433,0.0001573214,0.00001610647,0.993092,0.00001299713,0.004563956,0.0001652315],"study_design_scores_gemma":[0.003103747,0.001242872,0.00001327855,0.00005062933,0.00002105357,0.00000708731,0.0002264877,0.000166035,0.9750665,0.00002733927,0.01993314,0.0001417771],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.5150707,0.0002317754,0.4597279,0.00002527959,0.0002564226,0.02446005,0.0000100419,0.00001096803,0.0002068886],"genre_scores_gemma":[0.2655786,0.00002458284,0.7247268,0.0006958311,0.0007184226,0.006858319,0.00007909486,0.00007358508,0.001244772],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.2649989,"threshold_uncertainty_score":0.6259947,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03404001117065013,"score_gpt":0.5169112013292199,"score_spread":0.4828711901585697,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}