{"id":"W4411265387","doi":"10.1139/bcb-2025-0093","title":"A reliable Western blot workflow with improved dynamic range for the detection of myelin proteins in murine brain","year":2025,"lang":"en","type":"article","venue":"Biochemistry and Cell Biology","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Northern British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; Michael Smith Health Research BC; University of Northern British Columbia","keywords":"Western blot; Myelin; Workflow; Computational biology; Neuroscience; Cell biology; Biology; Molecular biology; Computer science; Chemistry; Biochemistry; Central nervous system; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.006682031,0.002350514,0.001408595,0.00273114,0.001131378,0.001565064,0.001785612,0.001258347,0.002974102],"category_scores_gemma":[0.003550425,0.00143068,0.001435102,0.001499746,0.0008847138,0.00118405,0.00123173,0.002983044,0.004079341],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008711556,"about_ca_system_score_gemma":0.001763596,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007356002,"about_ca_topic_score_gemma":0.001558288,"domain_scores_codex":[0.9940377,0.001094942,0.0008627627,0.001582001,0.002037153,0.0003854422],"domain_scores_gemma":[0.9968081,0.0004377121,0.0004420122,0.0006303812,0.001525749,0.0001560093],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001245518,0.00006604107,0.0001479986,0.0001846948,0.00002216081,0.00004456973,0.00006616992,0.0001873922,0.9932508,0.0003015524,0.0005670909,0.005036983],"study_design_scores_gemma":[0.00004906298,0.0003376764,0.002145688,0.00009714202,0.0001037879,0.0003829341,0.00003055555,0.00362033,0.9704705,0.0004145203,0.02226029,0.00008752773],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.05148182,0.001879797,0.9300306,0.0003609639,0.000455821,0.002802428,0.003205285,0.007533452,0.002249843],"genre_scores_gemma":[0.03924154,0.002412832,0.9404296,0.0002629262,0.0001069546,0.006879302,0.005850173,0.001202527,0.003614143],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.006682031,"threshold_uncertainty_score":0.0353384,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003272855190613216,"score_gpt":0.2319511676029881,"score_spread":0.2286783124123749,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}