{"id":"W4411373987","doi":"10.17504/protocols.io.4r3l2qb9jl1y/v3","title":"SARS-CoV-2 nsp3 macrodomain His-tagged expression and purification protocol for assay v2","year":2024,"lang":"en","type":"preprint","venue":"","topic":"SARS-CoV-2 and COVID-19 Research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Discovery Centre","funders":"National Institute of Allergy and Infectious Diseases; National Institutes of Health","keywords":"Protocol (science); Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); Coronavirus disease 2019 (COVID-19); Virology; Computational biology; Chromatography; Chemistry; Molecular biology; Biology; Medicine; Infectious disease (medical specialty); Internal medicine","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001169612,0.0021081,0.001628188,0.001679158,0.001515478,0.001184832,0.001874505,0.0008138453,0.03743347],"category_scores_gemma":[0.001070223,0.001378473,0.00117982,0.001423034,0.0005769413,0.0006736786,0.001284311,0.004001739,0.05335626],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008242257,"about_ca_system_score_gemma":0.001181221,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001970294,"about_ca_topic_score_gemma":0.00315493,"domain_scores_codex":[0.9985572,0.0002182976,0.0001490208,0.0002920183,0.0005501001,0.0002334817],"domain_scores_gemma":[0.9994732,0.00007250084,0.00002904621,0.0002084487,0.0001372344,0.0000795818],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0005032712,0.0002192141,0.0002853662,0.0005880455,0.0000522023,0.0001738092,0.0001819367,0.000297672,0.9510162,0.002317956,0.03194403,0.0124203],"study_design_scores_gemma":[0.0001278292,0.0002002609,0.001990235,0.00009414009,0.00006776713,0.0005843126,0.00006457468,0.001600222,0.7044491,0.001174685,0.2895463,0.0001005833],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.0996242,0.006081976,0.5687286,0.001971915,0.004082382,0.01739565,0.2178175,0.01690755,0.06739014],"genre_scores_gemma":[0.1017606,0.006148443,0.2909238,0.001064145,0.0004389503,0.01706925,0.4301148,0.006247927,0.146232],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.03743347,"threshold_uncertainty_score":0.1252275,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.09067384289263004,"score_gpt":0.4245476980999092,"score_spread":0.3338738552072792,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}