{"id":"W4411615101","doi":"10.1007/978-1-0716-4654-0_10","title":"Proximity Ligation Assay","year":2025,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":false,"ca_institutions":"McMaster University; Discovery Centre; Juravinski Cancer Centre","funders":"","keywords":"Proximity ligation assay; Ligation; Computational biology; Biology; Molecular biology; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002091935,0.003887821,0.003039982,0.004402016,0.003087983,0.002696363,0.002762342,0.002606628,0.03168241],"category_scores_gemma":[0.002122492,0.002109035,0.002993525,0.002831402,0.001604685,0.001296751,0.003949894,0.008284207,0.03209022],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001278036,"about_ca_system_score_gemma":0.001671302,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000627554,"about_ca_topic_score_gemma":0.001676567,"domain_scores_codex":[0.9886994,0.002542792,0.0006202094,0.00433268,0.002196574,0.00160833],"domain_scores_gemma":[0.9980476,0.0006467856,0.0001396635,0.0005672844,0.0002533992,0.0003452367],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006904834,0.0007469289,0.0006008105,0.00103755,0.0001629274,0.0003306806,0.0003351168,0.0003378226,0.9361457,0.009348314,0.01169852,0.03856508],"study_design_scores_gemma":[0.00009029971,0.0004681674,0.000936506,0.00008578496,0.0001066729,0.0005283115,0.00005621437,0.00165457,0.9061391,0.001243285,0.08862527,0.00006577185],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.0700309,0.004784677,0.8342502,0.001248537,0.001540769,0.00413148,0.01037954,0.01878982,0.05484404],"genre_scores_gemma":[0.2176503,0.004421958,0.5512398,0.001989443,0.0006619203,0.007899437,0.06059073,0.002082404,0.1534639],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.03168241,"threshold_uncertainty_score":0.1059882,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01092859260661219,"score_gpt":0.4189956136495653,"score_spread":0.4080670210429531,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}