{"id":"W4411615101","doi":"10.1007/978-1-0716-4654-0_10","title":"Proximity Ligation Assay","year":2025,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":false,"ca_institutions":"McMaster University; Discovery Centre; Juravinski Cancer Centre","funders":"","keywords":"Proximity ligation assay; Ligation; Computational biology; Biology; Molecular biology; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001113571,0.0001669836,0.0002011456,0.0001463165,0.00003870372,0.00000944946,0.0002887067,0.0003557805,0.000006200735],"category_scores_gemma":[0.0008715992,0.0001682722,0.00007457182,0.0002807042,0.0001752817,0.000003026415,0.0002189615,0.0001852122,0.000001836797],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003735819,"about_ca_system_score_gemma":0.00007573827,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001267044,"about_ca_topic_score_gemma":0.0000102965,"domain_scores_codex":[0.9980748,0.0008345873,0.0002707951,0.0004984862,0.00003587708,0.0002854354],"domain_scores_gemma":[0.9993285,0.00003981819,0.00006712307,0.0004701995,0.00006509059,0.00002928854],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002890662,0.00003555249,0.004348153,0.0000108731,0.00001518686,0.000002468921,0.000006377019,0.00001262539,0.9568397,0.006817485,0.0001037164,0.03177892],"study_design_scores_gemma":[0.0002084427,0.00009136621,0.0003736075,0.00001861836,0.000006298158,0.000002788301,0.000008778554,0.00007183241,0.9551348,0.02682995,0.01710409,0.0001494226],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.009203973,0.0009057279,0.9866573,0.0002224516,0.0001661284,0.0003179597,0.000003852084,0.00003933487,0.002483265],"genre_scores_gemma":[0.0138534,0.00008658946,0.9846772,0.0008628967,0.00002423771,0.0001139144,0.0000573137,0.00001620744,0.0003082664],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.0316295,"threshold_uncertainty_score":0.6861942,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01092859260661219,"score_gpt":0.4189956136495653,"score_spread":0.4080670210429531,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}