{"id":"W4411619808","doi":"10.1002/adhm.202501788","title":"Admixing of mRNA with Pre‐Formed Lipid Nanoparticles Containing a Slightly‐Cationic Ionizable Lipid Allows for Efficient mRNA Transfection In Vitro and In Vivo","year":2025,"lang":"en","type":"article","venue":"Advanced Healthcare Materials","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"European Research Council; Fonds Wetenschappelijk Onderzoek","keywords":"Messenger RNA; In vivo; Transfection; In vitro; Chemistry; Biophysics; Biochemistry; Biology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002897779,0.0003471958,0.0001759355,0.0001391026,0.000133515,0.0002198443,0.0001590103,0.0002978696,0.0006083145],"category_scores_gemma":[0.0002329368,0.0001313629,0.0002314391,0.00009048957,0.0002502612,0.0001744061,0.000187677,0.0005581444,0.0002731549],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003152586,"about_ca_system_score_gemma":0.0002715771,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003649662,"about_ca_topic_score_gemma":0.000557791,"domain_scores_codex":[0.9998435,0.00002489006,0.00001574772,0.00004516457,0.0000501261,0.0000205512],"domain_scores_gemma":[0.9998965,0.00003044821,0.0000281507,0.00001324735,0.00001740561,0.0000142505],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002620133,0.000009582581,0.00006041149,0.00001684208,0.000002096491,0.0000213571,0.000008547588,0.00004389006,0.9987547,0.00008105187,0.0000126785,0.0009625037],"study_design_scores_gemma":[0.000003151344,0.00008343313,0.0002063318,0.000001486865,0.00000458555,0.00003555657,0.000003865105,0.0003577964,0.9983669,0.00001505952,0.0009199714,0.000001875942],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9645004,0.001349627,0.03052404,0.0001689257,0.0001176522,0.0001125973,0.0001019085,0.0001525248,0.002972396],"genre_scores_gemma":[0.9729764,0.0009061256,0.02181384,0.0001178136,0.00002471015,0.00008017815,0.0002406165,0.00004930202,0.003790973],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006083145,"threshold_uncertainty_score":0.002287388,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008860717219442364,"score_gpt":0.2766436591777999,"score_spread":0.2677829419583575,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}