{"id":"W4411669538","doi":"10.17504/protocols.io.5qpvowjn9l4o/v1","title":"FLAG-tag mediated co-immunoprecipitation of phosphorylated Rab GTPases with cellular interacting proteins v1","year":2025,"lang":"en","type":"preprint","venue":"","topic":"Cellular transport and secretion","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Trinity College","funders":"Medical Research Council","keywords":"Rab; Immunoprecipitation; GTPase; Cell biology; Phosphorylation; Chemistry; Flag (linear algebra); Biology; Biochemistry; Gene; Mathematics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003332784,0.0009884485,0.0006008112,0.0004842396,0.0003390947,0.0005999382,0.0007664086,0.0003986843,0.004354323],"category_scores_gemma":[0.0002242577,0.0004178936,0.0004660815,0.0008070078,0.0002417391,0.0003411483,0.0006308315,0.001185194,0.003512461],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006458979,"about_ca_system_score_gemma":0.0003323311,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001506363,"about_ca_topic_score_gemma":0.003112969,"domain_scores_codex":[0.999421,0.00006511137,0.000031723,0.00014268,0.0001441812,0.0001953444],"domain_scores_gemma":[0.999878,0.00002889273,0.00001662288,0.00002678992,0.00002485818,0.00002475912],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002039881,0.00005190322,0.0001335194,0.00009012923,0.00002311721,0.00008102256,0.00002405511,0.00008789566,0.997285,0.000271026,0.0006933328,0.001055073],"study_design_scores_gemma":[0.00001925977,0.00006084646,0.002240498,0.000005936458,0.00001271816,0.0001783312,0.00001978374,0.001766482,0.9891684,0.00007260836,0.006445338,0.000009772054],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9339314,0.004332733,0.03365203,0.0008076133,0.0005915136,0.0002302086,0.007347255,0.001061819,0.0180455],"genre_scores_gemma":[0.8747749,0.002195568,0.0304468,0.0003979109,0.0001180796,0.0003067232,0.03084203,0.00052858,0.06038944],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004354323,"threshold_uncertainty_score":0.01456666,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008697022645409756,"score_gpt":0.2406374108070965,"score_spread":0.2319403881616868,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}