{"id":"W4412067191","doi":"10.1016/j.sbi.2025.103110","title":"Probing structural dynamics and interactions in macromolecular complexes with single-molecule force spectroscopy","year":2025,"lang":"en","type":"review","venue":"Current Opinion in Structural Biology","topic":"Force Microscopy Techniques and Applications","field":"Physics and Astronomy","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"Canadian Institutes of Health Research","keywords":"Force spectroscopy; Macromolecule; Molecule; Chemistry; Dynamics (music); Chemical physics; Spectroscopy; Molecular dynamics; Biophysics; Nanotechnology; Computational chemistry; Materials science; Physics; Biology; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.00004180849,0.0004548033,0.0009155969,0.0003066856,0.0001057983,0.00006542351,0.0002739086,0.00009588554,0.00004538479],"category_scores_gemma":[0.000003719363,0.0003550751,0.0001340406,0.0004423786,0.0001872703,0.00006853582,0.000193232,0.0006646915,0.000001278583],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001978185,"about_ca_system_score_gemma":0.000108682,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001347844,"about_ca_topic_score_gemma":0.00004404722,"domain_scores_codex":[0.9982905,0.0001045814,0.0005736302,0.0005986586,0.00004893191,0.0003837388],"domain_scores_gemma":[0.9992642,0.00008022114,0.0002952167,0.0002793467,0.00003228267,0.00004876198],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00002077408,0.00004795,0.002602453,0.006779702,0.00009240415,6.598339e-7,0.0001024348,0.000008018154,0.0003855149,0.1978409,0.00008993047,0.7920292],"study_design_scores_gemma":[0.003876944,0.0009965854,0.0008969302,0.1076732,0.0006820098,0.0002273025,0.0005889337,0.00594227,0.001219841,0.07474053,0.7967616,0.006393875],"study_design_candidate":"design_other","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.003986585,0.9812694,0.009099819,0.000055843,0.001331211,0.002514346,0.001045063,0.00007960399,0.0006181029],"genre_scores_gemma":[0.05773914,0.9138167,0.009527951,0.00001733515,0.000668072,0.001484568,0.01642751,0.0001428341,0.0001759053],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.7966716,"threshold_uncertainty_score":0.9998901,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03123332503102513,"score_gpt":0.3929561160578463,"score_spread":0.3617227910268211,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}