{"id":"W4413106926","doi":"10.1038/s41598-025-13532-z","title":"Miniaturized scalable arrayed CRISPR screening in primary cells enables discovery at the single donor resolution","year":2025,"lang":"en","type":"article","venue":"Scientific Reports","topic":"Electrowetting and Microfluidic Technologies","field":"Engineering","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University; McGill University","funders":"MEDTEQ+; Natural Sciences and Engineering Research Council of Canada; Mitacs; Canadian Institutes of Health Research; Massachusetts Life Sciences Center","keywords":"CRISPR; Electroporation; Cas9; Genome editing; Computational biology; Genome engineering; Functional genomics; Primary cell; Scalability; Computer science; Biology; Genomics; Genome; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000426975,0.0003920124,0.0005424771,0.0003125669,0.0001883129,0.0005448729,0.00039821,0.000462062,0.001782519],"category_scores_gemma":[0.0004072234,0.0002240839,0.0003289892,0.0001504365,0.0003437177,0.0002955488,0.0005768558,0.0006286181,0.0007193472],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004340995,"about_ca_system_score_gemma":0.0003632538,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003075254,"about_ca_topic_score_gemma":0.000928817,"domain_scores_codex":[0.9996837,0.00003311959,0.0000227374,0.0001168926,0.0001041539,0.00003934279],"domain_scores_gemma":[0.9997712,0.00009713836,0.00003624109,0.00004005383,0.00002751188,0.0000278326],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001675393,0.000009864649,0.0001205632,0.00002240017,0.000004922115,0.00002460253,0.000007252554,0.0003112237,0.9962336,0.0002114008,0.0001135578,0.002923974],"study_design_scores_gemma":[0.00000641215,0.00007000896,0.0006734092,0.000003504934,0.000008344075,0.00009754259,0.000007556036,0.002802573,0.9935431,0.0001772197,0.002603903,0.000006370957],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.4933253,0.00156981,0.4922491,0.0005428542,0.0001553604,0.000474636,0.003367087,0.003693453,0.004622382],"genre_scores_gemma":[0.7443391,0.001610145,0.2461892,0.0002524746,0.00003514513,0.000552943,0.001956814,0.0001874224,0.004876846],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001782519,"threshold_uncertainty_score":0.005963087,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006276180016647375,"score_gpt":0.1939741569649568,"score_spread":0.1876979769483095,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}