{"id":"W4413411072","doi":"10.1002/bit.70043","title":"Endogenous Retrovirus‐Like Particle‐Deficient CHO Cells Can be Generated by CRISPR or shRNA and Enriched Based on Cell‐Surface Expression of Retroviral Envelope Protein","year":2025,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada","funders":"National Research Council Canada","keywords":"Chinese hamster ovary cell; Retrovirus; CRISPR; Biology; Murine leukemia virus; Endogenous retrovirus; Cell culture; Viral envelope; Virology; Cell biology; Virus; Recombinant DNA; Small hairpin RNA; Viral vector; Capsid; Molecular biology; Gene; Genetics; Genome","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001019905,0.0002338904,0.0002106848,0.00008764557,0.000134848,0.00002081102,0.0001361594,0.0004546441,0.000008700742],"category_scores_gemma":[0.00004073369,0.0001912829,0.00004470207,0.0002223814,0.0001351818,0.00000416592,0.0001459576,0.0001756462,4.639327e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001871089,"about_ca_system_score_gemma":0.00005626918,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003445905,"about_ca_topic_score_gemma":0.000004922754,"domain_scores_codex":[0.9988727,0.00004236959,0.0002396965,0.0004538135,0.00009180987,0.0002996384],"domain_scores_gemma":[0.9994612,0.00001405996,0.00007953542,0.0003288132,0.00003653125,0.00007981133],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002324853,0.0001139824,0.0004226154,0.00009726522,0.00002057494,0.000005097102,0.0000138824,0.001348152,0.9963412,0.00002609573,0.0009245632,0.0004541135],"study_design_scores_gemma":[0.0007911475,0.0007542779,0.00006842076,0.00005428144,0.0000223473,0.000002880219,0.00004282058,0.002591414,0.991954,0.000003981829,0.00350176,0.0002126994],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9936762,0.001929277,0.003427109,0.0002583244,0.0001418245,0.0003734668,0.00007791324,0.00006504112,0.00005085465],"genre_scores_gemma":[0.9978488,0.0003963721,0.001104633,0.0002500093,0.00001807935,0.00003770669,0.00003734115,0.00001867527,0.0002883798],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004387204,"threshold_uncertainty_score":0.7800291,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008516880894238757,"score_gpt":0.2153143853068364,"score_spread":0.2067975044125976,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}