{"id":"W4413422955","doi":"10.3791/68411","title":"A GFP Complementation-based Dual-expression System for Assessing Cell-Cell Contact Mediated by Cytonemes in Live <em>Drosophila</em> Wing Imaginal Discs","year":2025,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Morphological variations and asymmetry","field":"Mathematics","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; Montreal Clinical Research Institute","funders":"","keywords":"Imaginal disc; Cell biology; Complementation; Drosophila (subgenus); Biology; Green fluorescent protein; Drosophila melanogaster; Dual (grammatical number); Cell; Over expression; Phenotype; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006500029,0.001037397,0.00052254,0.000787878,0.0005152441,0.0005872717,0.001557014,0.0009624716,0.002696821],"category_scores_gemma":[0.0002251481,0.0005003953,0.0004893013,0.0004761719,0.0009146516,0.0006297634,0.0008299865,0.002095425,0.00167058],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009411151,"about_ca_system_score_gemma":0.0005094723,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008681088,"about_ca_topic_score_gemma":0.001574763,"domain_scores_codex":[0.9993775,0.00007946218,0.00005676025,0.0001996599,0.0002233187,0.00006340989],"domain_scores_gemma":[0.999551,0.00008361138,0.0001177162,0.00009526072,0.00005194476,0.000100393],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001344779,0.00001287887,0.00002137762,0.00002067866,0.000001790119,0.000009954077,0.000008471442,0.00002046084,0.9992796,0.000117775,0.00002223506,0.0004714325],"study_design_scores_gemma":[0.000009640655,0.00004907167,0.0005319475,0.000003861946,0.000007561966,0.000114253,0.000009206375,0.0007698034,0.996799,0.0000246793,0.001675733,0.00000527874],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6815627,0.001322246,0.302461,0.0004326664,0.0001588972,0.001061006,0.00376715,0.002226996,0.007007268],"genre_scores_gemma":[0.6883565,0.00170394,0.2883175,0.0002295532,0.00003786742,0.001871784,0.005739786,0.0007347707,0.01300833],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002696821,"threshold_uncertainty_score":0.009021759,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03943672125565748,"score_gpt":0.4074482234427915,"score_spread":0.368011502187134,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}