{"id":"W4413470426","doi":"10.1016/j.xpro.2025.104044","title":"Protocol for dual-optical mapping of voltage and calcium sensors in human pluripotent stem cell-derived cardiomyocytes","year":2025,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Neuroscience and Neural Engineering","field":"Neuroscience","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"Stem Cell Network; University of Waterloo; University of Toronto; McMaster University; University Health Network","funders":"Canada Research Chairs; University of California Berkeley","keywords":"Induced pluripotent stem cell; Dual (grammatical number); Protocol (science); Human Induced Pluripotent Stem Cells; Voltage; Cell biology; Computer science; Neuroscience; Biology; Electrical engineering; Engineering; Medicine; Embryonic stem cell; Biochemistry; Pathology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006823806,0.001253653,0.000555794,0.0009241741,0.001089661,0.0003954389,0.001295088,0.0005667636,0.01119264],"category_scores_gemma":[0.0004741588,0.0008103043,0.000606497,0.0007542277,0.0004636224,0.0004230024,0.0006495512,0.002162254,0.00394045],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00053413,"about_ca_system_score_gemma":0.001097458,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006013107,"about_ca_topic_score_gemma":0.002695931,"domain_scores_codex":[0.9993228,0.0001188127,0.0001093373,0.0001532061,0.0002171298,0.00007865563],"domain_scores_gemma":[0.9995158,0.000138306,0.00004344545,0.0001121728,0.000123968,0.00006629684],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0001150115,0.00007992628,0.00007926134,0.0001822871,0.00001578673,0.0001543985,0.00008669878,0.0002299204,0.9908096,0.001415916,0.001390101,0.005441135],"study_design_scores_gemma":[0.0001408346,0.0003759661,0.001834207,0.00004634756,0.00004585878,0.000699515,0.00004542781,0.002604688,0.9428449,0.000910052,0.05040123,0.00005104201],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.1161333,0.002597552,0.8372509,0.0008830898,0.0007181808,0.01082517,0.00730516,0.002943266,0.02134348],"genre_scores_gemma":[0.1698685,0.00437715,0.7349426,0.0007669597,0.0001545393,0.04334292,0.01256471,0.0006454964,0.03333713],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.01119264,"threshold_uncertainty_score":0.03744316,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07873367145985144,"score_gpt":0.351875296206273,"score_spread":0.2731416247464216,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}