{"id":"W4413473455","doi":"10.1038/s41467-025-63164-0","title":"Structure-guided engineering of type I-F CASTs for targeted gene insertion in human cells","year":2025,"lang":"en","type":"article","venue":"Nature Communications","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"Tornado Spectral Systems (Canada)","funders":"Common Fund; National Institute of Biomedical Imaging and Bioengineering; National Institute of General Medical Sciences; Irving Medical Center, Columbia University; U.S. Department of Health and Human Services; National Institutes of Health; National Human Genome Research Institute; St. Jude Children's Research Hospital","keywords":"Gene; Computational biology; Biology; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001972275,0.0003027346,0.0001806035,0.0001756988,0.0001395343,0.0003531304,0.0002442261,0.0003360076,0.0008256677],"category_scores_gemma":[0.0001829417,0.0001703801,0.0003043953,0.0001241939,0.0002378157,0.0001071542,0.0002138702,0.0004334009,0.0003631049],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003988213,"about_ca_system_score_gemma":0.0003349188,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001118107,"about_ca_topic_score_gemma":0.002165857,"domain_scores_codex":[0.9998932,0.00001657498,0.000006627341,0.00002090743,0.00004324232,0.00001937597],"domain_scores_gemma":[0.9999056,0.00001538285,0.00002879299,0.00001698205,0.00001006026,0.00002314866],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004074784,0.00001408173,0.000169285,0.00002175416,0.000006660669,0.00007074032,0.00001619459,0.0007114341,0.9964538,0.0005226249,0.00006667738,0.00190603],"study_design_scores_gemma":[0.000007501979,0.00007307444,0.0004494438,0.000003389789,0.000007170559,0.0001646719,0.00001266839,0.00225458,0.9942819,0.00006487622,0.002676409,0.000004406365],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8980353,0.0006992961,0.09430728,0.0001788209,0.00007425997,0.0001870296,0.0008819821,0.0005650605,0.005070967],"genre_scores_gemma":[0.9566471,0.0005307667,0.03883952,0.00005314386,0.000006155757,0.00004066195,0.0004408018,0.0000842986,0.003357437],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001118107,"threshold_uncertainty_score":0.002893627,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01030549389990176,"score_gpt":0.3370663779249291,"score_spread":0.3267608840250273,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}