{"id":"W4413636357","doi":"10.1021/acs.biochem.5c00172","title":"Stuffed Epimerase Domains of Pyochelin Biosynthesis are Defunct Methyltransferases","year":2025,"lang":"en","type":"article","venue":"Biochemistry","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Wilfrid Laurier University","funders":"Division of Chemistry; National Institute of General Medical Sciences; Natural Sciences and Engineering Research Council of Canada; Wilfrid Laurier University","keywords":"Methyltransferase; Biosynthesis; Chemistry; Biochemistry; Enzyme; Gene; Methylation","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008848533,0.000325056,0.000150946,0.0001178323,0.0001058374,0.000217509,0.0001271619,0.0001603492,0.001073624],"category_scores_gemma":[0.0001419757,0.0001120598,0.0001543107,0.0001282908,0.0001558073,0.0002274893,0.0001694502,0.0003728033,0.0003455066],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002379287,"about_ca_system_score_gemma":0.0002135208,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007411968,"about_ca_topic_score_gemma":0.0009932352,"domain_scores_codex":[0.9998996,0.0000142884,0.000008283234,0.00002440443,0.00003042799,0.00002308609],"domain_scores_gemma":[0.9998984,0.0000193289,0.0000295175,0.00001817085,0.00001565956,0.00001884546],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004906848,0.000005705944,0.0003188606,0.00001313632,0.000001807388,0.00005087646,0.00001205739,0.00005657381,0.9983498,0.00007044028,0.000005739103,0.001065974],"study_design_scores_gemma":[0.00000310934,0.00007720166,0.003791704,0.000002063279,0.000003994482,0.0002097484,0.00002045519,0.0002748512,0.9944242,0.00003561337,0.001153593,0.000003541029],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9952121,0.0002989218,0.002608463,0.00002694749,0.000005902801,0.00001489123,0.000224878,0.00003824455,0.001569634],"genre_scores_gemma":[0.9946445,0.0001966834,0.002873163,0.00001823422,0.000002278325,0.000009558156,0.0005531631,0.00001435999,0.001688089],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001073624,"threshold_uncertainty_score":0.003591657,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009323409968317709,"score_gpt":0.2573404340002343,"score_spread":0.2480170240319166,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}