{"id":"W4413744848","doi":"10.1128/spectrum.00998-25","title":"Development of a rapid and sensitive RPA-CRISPR/Cas12a-based assay for the detection of <i>Brucella melitensis</i>","year":2025,"lang":"en","type":"article","venue":"Microbiology Spectrum","topic":"Brucella: diagnosis, epidemiology, treatment","field":"Veterinary","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Infection and Immunity","funders":"","keywords":"Brucella melitensis; CRISPR; Brucella; Biology; Brucellosis; Brucella abortus; Computational biology; Microbiology; Virology; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001153293,0.0002958502,0.0006782569,0.0001941597,0.000197967,0.000005843901,0.0001659139,0.0002996023,0.00002474494],"category_scores_gemma":[0.0002687644,0.0002212355,0.0001874098,0.0001969793,0.0004560607,0.00002034172,0.0001224409,0.0001806328,0.000009383832],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001519202,"about_ca_system_score_gemma":0.000148881,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001491619,"about_ca_topic_score_gemma":0.0004617697,"domain_scores_codex":[0.9978454,0.0004620318,0.0007174293,0.000523683,0.00003754438,0.0004139646],"domain_scores_gemma":[0.9960818,0.002974167,0.0003677534,0.0004170188,0.0001219655,0.00003724167],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0009934793,0.0001773202,0.001252821,0.0001162398,0.000622383,0.00000838403,0.0002767584,0.00001046062,0.9862126,0.0003345558,0.001088807,0.008906174],"study_design_scores_gemma":[0.001550929,0.0005693707,0.01110105,0.00008475038,0.0002336149,0.00008064916,0.0002975966,0.0002065862,0.9592687,0.0002364784,0.02617488,0.0001954064],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9646609,0.004077275,0.02273491,0.005423895,0.0007512046,0.001650109,0.0002267741,0.00007573821,0.0003991419],"genre_scores_gemma":[0.9905302,0.0001131829,0.008078712,0.0009522151,0.00003424438,0.0001326562,0.0000559947,0.00002214048,0.00008063402],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02694394,"threshold_uncertainty_score":0.9021723,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03067433274255649,"score_gpt":0.2993485958375237,"score_spread":0.2686742630949672,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}