{"id":"W4413744848","doi":"10.1128/spectrum.00998-25","title":"Development of a rapid and sensitive RPA-CRISPR/Cas12a-based assay for the detection of <i>Brucella melitensis</i>","year":2025,"lang":"en","type":"article","venue":"Microbiology Spectrum","topic":"Brucella: diagnosis, epidemiology, treatment","field":"Veterinary","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Infection and Immunity","funders":"","keywords":"Brucella melitensis; CRISPR; Brucella; Biology; Brucellosis; Brucella abortus; Computational biology; Microbiology; Virology; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001504274,0.001298957,0.000994386,0.0009845176,0.0003719995,0.001106018,0.001125311,0.001397846,0.001511353],"category_scores_gemma":[0.002004484,0.0006728329,0.0008361919,0.0003858708,0.0006980854,0.0004421257,0.0007314992,0.001494304,0.001565337],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005261226,"about_ca_system_score_gemma":0.0006700283,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009350575,"about_ca_topic_score_gemma":0.001427887,"domain_scores_codex":[0.997338,0.0005217378,0.000243215,0.0008034821,0.0009058661,0.0001876914],"domain_scores_gemma":[0.9985697,0.0004525215,0.0003588065,0.0001435535,0.0003552266,0.0001201516],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000334311,0.00003557684,0.0005857577,0.0001331552,0.00001774246,0.00003671972,0.00002232069,0.0001783877,0.9946612,0.0001031957,0.000262154,0.003930395],"study_design_scores_gemma":[0.00001420087,0.0002393819,0.001813356,0.00002772267,0.00003944804,0.0002882981,0.00001947029,0.004335684,0.9893678,0.00009232136,0.003728486,0.00003392978],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3589398,0.004287465,0.6132362,0.001018645,0.0005770107,0.001099969,0.004312909,0.008887408,0.007640514],"genre_scores_gemma":[0.6535628,0.001507022,0.3322871,0.0006389379,0.00009461663,0.001051842,0.004271632,0.0003271367,0.006258855],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001511353,"threshold_uncertainty_score":0.007955432,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03067433274255649,"score_gpt":0.2993485958375237,"score_spread":0.2686742630949672,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}