{"id":"W4413801833","doi":"10.1016/j.xpro.2025.104049","title":"Protocol for plasma membrane enrichment by ultracentrifugation for mass spectrometry of cell lines, xenografts, and patient tumors","year":2025,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Mass Spectrometry Techniques and Applications","field":"Chemistry","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canada's Michael Smith Genome Sciences Centre","funders":"Eunice Kennedy Shriver National Institute of Child Health and Human Development; Hyundai Hope On Wheels; National Cancer Institute; Stand Up To Cancer; National Institutes of Health; BC Cancer Foundation; Foundation for the National Institutes of Health; Cancer Research UK; Michael Smith Health Research BC; Mark Foundation For Cancer Research; Alex's Lemonade Stand Foundation for Childhood Cancer; W. W. Smith Charitable Trust; Osteosarcoma Institute; St. Baldrick's Foundation","keywords":"Ultracentrifuge; Mass spectrometry; Chemistry; Chromatography; Membrane; Plasma; Biochemistry; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001566652,0.0002295441,0.0003161001,0.0001277061,0.0001242123,0.00004509416,0.0002056858,0.0001320069,0.0002847356],"category_scores_gemma":[0.00005241307,0.0002143025,0.0001102923,0.0002759723,0.00005111434,0.00005914014,0.00004029576,0.0001204737,5.275775e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001136561,"about_ca_system_score_gemma":0.00008758673,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009943446,"about_ca_topic_score_gemma":0.000001003834,"domain_scores_codex":[0.9984852,0.00001284326,0.0005776637,0.0004158003,0.000191652,0.0003168416],"domain_scores_gemma":[0.9989012,0.000146757,0.0003539745,0.0003685174,0.0001564691,0.00007311621],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004942253,0.0006342211,0.0002980733,0.004633396,0.00003348368,2.057869e-7,0.00003752931,0.000002115078,0.9757057,0.007121362,0.007809423,0.003230203],"study_design_scores_gemma":[0.002225429,0.000350181,0.00001026643,0.0001951193,0.00001539591,4.431933e-7,0.00005298358,0.0003590196,0.7915158,0.003784022,0.2013322,0.0001592101],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"protocol","genre_gemma":"protocol","genre_scores_codex":[0.003475052,0.000006410733,0.01492056,0.0002602543,0.000008154339,0.9755432,0.0006751806,0.000144176,0.004967008],"genre_scores_gemma":[0.01047651,0.000001259778,0.06833379,0.00002800639,0.00003667106,0.9200596,0.0000937068,0.00002542711,0.0009450497],"genre_candidate":"protocol","genre_consensus":"protocol","teacher_disagreement_score":0.1935228,"threshold_uncertainty_score":0.8739004,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01265047046724243,"score_gpt":0.306969380315276,"score_spread":0.2943189098480336,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}