{"id":"W4414028236","doi":"10.1101/2025.09.03.674080","title":"Tri-Functional CRISPR Screen Reveals Overexpression of <i>QDR2</i> and <i>QDR3</i> Transporters Increase Fumaric Acid Production in <i>Kluyveromyces marxianus</i>","year":2025,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University","funders":"Fonds de recherche du Québec – Nature et technologies; Natural Sciences and Engineering Research Council of Canada; Concordia University; Genome Canada","keywords":"Kluyveromyces marxianus; CRISPR; Kluyveromyces; Transporter; Fumaric acid; Production (economics); Chemistry; Yeast; Food science; Biochemistry; Gene; Saccharomyces cerevisiae; Economics; Microeconomics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002178647,0.0005897249,0.0004557358,0.0004019739,0.0002042232,0.0004191584,0.0004048558,0.000391429,0.001880112],"category_scores_gemma":[0.0001545889,0.0002199502,0.0004947817,0.000280673,0.0002485631,0.0001489315,0.0004995891,0.0005677573,0.0008916653],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004608622,"about_ca_system_score_gemma":0.0002482822,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002027559,"about_ca_topic_score_gemma":0.002404546,"domain_scores_codex":[0.9996799,0.00002987498,0.00005138535,0.00007286813,0.0001021926,0.00006377859],"domain_scores_gemma":[0.9998263,0.00002802855,0.00005605612,0.00002394555,0.00001734376,0.00004842087],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004671219,0.00001407626,0.0001598425,0.00002749213,0.000005957617,0.00006056958,0.00001178615,0.00007337333,0.9989324,0.00005560022,0.0000548512,0.0005573116],"study_design_scores_gemma":[0.0000183745,0.0001253776,0.004848559,0.000009267192,0.00003976261,0.0002258796,0.00005905905,0.001683609,0.9895684,0.000045311,0.003359397,0.00001698309],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9822778,0.000497223,0.009046068,0.0002126602,0.00005910875,0.00008599666,0.003339441,0.001083165,0.003398545],"genre_scores_gemma":[0.9890125,0.0002571217,0.003996429,0.000098615,0.00000477994,0.00004901792,0.002202498,0.0001463932,0.004232612],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002027559,"threshold_uncertainty_score":0.006289601,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006923894666217551,"score_gpt":0.1956257148193158,"score_spread":0.1887018201530983,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}