{"id":"W4414072266","doi":"10.2174/0109298665393672250715000125","title":"Essential Role of Non-Conserved α4-His <sup>178</sup> in Stabilizing the α4-α5 Hairpin and Biotoxicity of the Cry4Aa Mosquitocidal Protein","year":2025,"lang":"en","type":"article","venue":"Protein and Peptide Letters","topic":"Insect Resistance and Genetics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Innovation Cluster (Canada)","funders":"","keywords":"Structural integrity; Protein stability; Membrane; Hydrogen bond; Structural stability; Membrane protein","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002888774,0.0001695952,0.0002053764,0.00004740651,0.0001112739,0.00002582727,0.0002445753,0.0001299534,0.000005094433],"category_scores_gemma":[0.00007033688,0.0001193525,0.00008661387,0.0001559432,0.0004037903,0.000005773737,0.0002063009,0.0001693886,2.211183e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001080467,"about_ca_system_score_gemma":0.00007828712,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004922062,"about_ca_topic_score_gemma":0.0005825513,"domain_scores_codex":[0.9988773,0.0001469509,0.0003100982,0.000294277,0.0001351767,0.0002362335],"domain_scores_gemma":[0.9994016,0.00001968222,0.000125806,0.000378299,0.0000460854,0.00002849261],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001535086,0.00003465595,0.01090229,0.0001998763,0.00003863813,8.14763e-7,0.0003372687,0.00009073613,0.9873653,0.00006895338,0.0001361212,0.0006718209],"study_design_scores_gemma":[0.0005909046,0.00008095851,0.01458536,0.00017767,0.00001689912,0.000001465096,0.0003264575,0.0002591134,0.9826066,0.000243042,0.0009819516,0.0001295348],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.995196,0.0009123346,0.0001553657,0.002392397,0.00001948808,0.001092257,0.00001933123,0.000003685236,0.0002091574],"genre_scores_gemma":[0.99857,0.00003686898,0.0004449395,0.0006140327,0.00005317467,0.0001047751,0.000005326462,0.00001149289,0.0001593832],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004758674,"threshold_uncertainty_score":0.4867054,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.002967500192238043,"score_gpt":0.2062996478674219,"score_spread":0.2033321476751838,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}