{"id":"W4414158242","doi":"10.1101/2025.09.08.674725","title":"Optimized CRISPR/Cas9 Electroporation and Single Cell Cloning Protocol for Generating Pure Cellular Models in Human Immortalized Myoblasts","year":2025,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"Courtois Foundation; Muscular Dystrophy Canada","keywords":"Electroporation; Cell sorting; Sanger sequencing; Transfection; C2C12; Genome editing; Cell culture; Myocyte; Cloning (programming); HEK 293 cells","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002095939,0.0007152655,0.0007427669,0.0007499916,0.0006491328,0.000874726,0.0007907607,0.0007222869,0.001600877],"category_scores_gemma":[0.001381594,0.0004968207,0.0007160587,0.0004520189,0.0003923115,0.0003163235,0.0006502179,0.001210425,0.001868143],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006876453,"about_ca_system_score_gemma":0.001054688,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001147252,"about_ca_topic_score_gemma":0.002408224,"domain_scores_codex":[0.9981592,0.000275844,0.0003157721,0.0004194243,0.0007049959,0.0001246552],"domain_scores_gemma":[0.999238,0.0001720896,0.000113878,0.00016968,0.0002486134,0.00005770936],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00004783988,0.00002689105,0.0002248395,0.00008571515,0.00001196265,0.00008999478,0.00005763452,0.0008873582,0.9932366,0.0002567082,0.0003853139,0.004689158],"study_design_scores_gemma":[0.000007128639,0.00008472006,0.0007903457,0.00001544114,0.00002528658,0.0001651564,0.00001841858,0.003903893,0.9893801,0.0001049911,0.005481401,0.0000232024],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.2894204,0.00139388,0.6921513,0.0003989202,0.0002617353,0.001698339,0.003925891,0.005762808,0.004986748],"genre_scores_gemma":[0.401778,0.001455425,0.5783485,0.0002389938,0.0000279539,0.002556504,0.006280823,0.001390983,0.007922694],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.002095939,"threshold_uncertainty_score":0.0110845,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01318728626969423,"score_gpt":0.2772181693030955,"score_spread":0.2640308830334012,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}